GSPT1 Protein, Human (His)

GSPT1 Protein, Human (His) is the recombinant human-derived GSPT1 protein, expressed by E. coli, with C-His tag.

eRF3a is the GTPase component of the eRF1-eRF3-GTP ternary complex, which mediates translation termination in response to stop codons UAA, UAG, and UGA. GSPT1/ERF3A delivers ETF1/ERF1 to stop codons: The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site, and GTP hydrolysis by GSPT1/ERF3A induces a conformational change leading to its dissociation, allowing ETF1/ERF1 to fully occupy the A-site. Additionally, eRF3a is part of the transient SURF complex that recruits UPF1 to stalled ribosomes during nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. It is also required for SHFL-mediated translation termination, which inhibits programmed ribosomal frameshifting (-1PRF) in viral and cellular mRNAs. by similar: Its functions are supported by studies on complex binding, conformational dynamics, and roles in NMD and frameshifting regulation.

Human

E. coli

C-His

P15170-1 (E2-D499)

2935

Partial

Approximately 65 kDa,based on SDS-PAGE under reducing conditions.

Greater than 90% as determined by reducing SDS-PAGE.

Solution

Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with glycerol as protectant.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.