2. Recombinant Proteins
  3. Viral Proteins
  4. Dengue Virus Proteins
  5. DENV E Proteins
  6. E/Envelope Protein, Dengue virus 2 (399a.a, T76R, Q77E, W101R, L107R, sf9, His)

E/Envelope Protein, Dengue virus 2 (399a.a, T76R, Q77E, W101R, L107R, sf9, His)

Cat. No.: HY-P74195
COA
SDS
Handling Instructions Technical Support

E/Envelope Protein, Dengue virus 2 (399a.a, T76R, Q77E, W101R, L107R, sf9, His) is the recombinant Virus-derived E/Envelope protein, expressed by Sf9 insect cells, with C-His labeled tag.

For research use only. We do not sell to patients.

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Description

E/Envelope Protein, Dengue virus 2 (399a.a, T76R, Q77E, W101R, L107R, sf9, His) is the recombinant Virus-derived E/Envelope protein, expressed by Sf9 insect cells, with C-His labeled tag.
Background

E/Envelope acts as a chaperone for the envelope protein E during intracellular virion assembly by masking and inactivating the fusion peptide of envelope protein E. prM is the only viral peptide matured by host furin in the trans-Golgi network, likely to prevent catastrophic activation of viral fusion activity in the acidic Golgi compartment before virion release. prM-E cleavage is inefficient, resulting in many virions being only partially matured. These uncleaved prM may contribute to immune evasion.
Species

Virus
Source

Sf9 insect cells
Tag

C-His
Accession

AAC59274.1 (M281-G679, T76R, Q77E, W101R, L107R)
Gene ID

/
Protein Length

Partial
Synonyms
E Protein; DENV; Dengue virus (DENV) (type 2, strain New Guinea C/PUO-218 hybrid) E / Envelope Protein (His Tag)
Predicted Molecular Mass
45.7 kDa
Molecular Weight

45.7 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
SDS (251 KB)

COA (221 KB)

Handling Instructions (2659 KB)

E/Envelope Protein, Dengue virus 2 (399a.a, T76R, Q77E, W101R, L107R, sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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E/Envelope Protein, Dengue virus 2 (399a.a, T76R, Q77E, W101R, L107R, sf9, His)
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