DDB1 Protein, Human (sf9, His)

DDB1 Protein, Human (sf9, His) is the recombinant human-derived DDB1, expressed by sf9 insect cells , with His labeled tag.

DDB1 is a multifunctional protein involved in both DNA repair and protein ubiquitination. As a core component of the UV-DDB complex, it recognizes UV-induced DNA damage (e.g., cyclobutane pyrimidine dimers CPD, 6-4 photoproducts 6-4 PP) and recruits nucleotide excision repair (NER) pathway proteins. Additionally, DDB1 serves as a scaffold for various DCX (DDB1-CUL4-X-box) E3 ubiquitin ligase complexes, where substrate-specific adaptors (e.g., DDB2, DTL, ERCC8) determine target specificity—mediating ubiquitination of histones (H2A/H3/H4), XPC, CRY1, etc., to regulate DNA damage response, transcription-coupled repair (TCR), circadian rhythms (via CRY1 degradation), and other processes. By similarity, DDB1 also stabilizes FOXO1 to promote gluconeogenesis, maintains oocytes via TET dioxygenases, and supports zygotic genome activation during early development.

Human

Sf9 insect cells

N-His

Q16531-1 (S2-H1140)

1642

Full Length of Isoform-1 Mature Protein

128.9 KDa

Greater than 90% as determined by reducing SDS-PAGE

Solution

Supplied as a 0.2 μm filtered solution of 50 mM HEPES (pH7.5), 200 mM NaCl, 20% glycerol, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.