ACE2 Protein, Mouse (HEK293, His)

ACE2 protein is an important carboxypeptidase in the renin-angiotensin system, which can regulate blood volume and vascular resistance and maintain cardiovascular homeostasis. It converts angiotensin I to the antihypertrophic angiotensin 1-9 and angiotensin II to the vasodilatory angiotensin 1-7, thereby opposing vasoconstriction. ACE2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ACE2 protein, expressed by HEK293, with C-His labeled tag.

Angiotensin-converting enzyme ACE2 is a metallopeptidase. As an important counter-regulatory carboxypeptidase of the renin-angiotensin system, it converts angiotensin I into angiotensin 1-9, which is anti-cardiac hypertrophy, and angiotensin II into angiotensin 1-7, which has vasodilatory and anti-proliferative properties, thereby counteracting the contractile effect of angiotensin II, regulating blood volume, systemic vascular resistance and cardiovascular homeostasis. At the same time, as a binding partner of the amino acid transporter SLC6A19, it regulates its cell surface transport and activity, participates in amino acid transport, and can regulate a variety of vasoactive peptides such as neurotensin and kinesin, as well as biological peptides such as apelins and casomorphin. It is a key player in cardiovascular disease and amino acid homeostasis. In addition, ACE2 is also a functional receptor for SARS-CoV and the new coronavirus (SARS-CoV-2). It can be immunoprecipitated by the S1 domain of SARS-CoV and promote viral replication. Its large presence in human lung and small intestinal epithelial cells provides a possible entry route for the virus. It is also an important regulator of cardiac function and blood pressure control, and may function as a natural counterpart of ACE1.

Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH. The specific activity is ≤462.95 pmol/min/µg, as measured under the described conditions.

Mouse

HEK293

C-His

Q8R0I0-1 (Q18-T740)

70008  [NCBI]

Extracellular Domain

QSLTEENAKTFLNNFNQEAEDLSYQSSLASWNYNTNITEENAQKMSEAAAKWSAFYEEQSKTAQSFSLQEIQTPIIKRQLQALQQSGSSALSADKNKQLNTILNTMSTIYSTGKVCNPKNPQECLLLEPGLDEIMATSTDYNSRLWAWEGWRAEVGKQLRPLYEEYVVLKNEMARANNYNDYGDYWRGDYEAEGADGYNYNRNQLIEDVERTFAEIKPLYEHLHAYVRRKLMDTYPSYISPTGCLPAHLLGDMWGRFWTNLYPLTVPFAQKPNIDVTDAMMNQGWDAERIFQEAEKFFVSVGLPHMTQGFWANSMLTEPADGRKVVCHPTAWDLGHGDFRIKMCTKVTMDNFLTAHHEMGHIQYDMAYARQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLPSDFQEDSETEINFLLKQALTIVGTLPFTYMLEKWRWMVFRGEIPKEQWMKKWWEMKREIVGVVEPLPHDETYCDPASLFHVSNDYSFIRYYTRTIYQFQFQEALCQAAKYNGSLHKCDISNSTEAGQKLLKMLSLGNSEPWTKALENVVGARNMDVKPLLNYFQPLFDWLKEQNRNSFVGWNTEWSPYADQSIKVRISLKSALGANAYEWTNNEMFLFRSSVAYAMRKYFSIIKNQTVPFLEEDVRVSDLKPRVSFYFFVTSPQNVSDVIPRSEVEDAIRMSRGRINDVFGLNDNSLEFLGIHPTLEPPYQPPVT

85-110 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 25 mM Tris-HCL, 150 mM NaCl, 1 mM ZnCl2, pH 7.4 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Hamming I, et, al. Tissue distribution of ACE2 protein, the functional receptor for SARS coronavirus. A first step in understanding SARS pathogenesis. J Pathol. 2004 Jun;203(2):631-7.  [Content Brief]

  [2]. Yang J, et, al. Molecular interaction and inhibition of SARS-CoV-2 binding to the ACE2 receptor. Nat Commun. 2020 Sep 11;11(1):4541.  [Content Brief]