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  4. ABCB1 Protein, Human (HEK293, Flag)

ABCB1 Protein, Human (HEK293, Flag) is the recombinant human-derived ABCB1, expressed by HEK293 , with tag Free labeled tag.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

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10 μg In-stock
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100 μg   Get quote  
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Top Publications Citing Use of Products

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

ABCB1 Protein, Human (HEK293, Flag) is the recombinant human-derived ABCB1, expressed by HEK293 , with tag Free labeled tag.
Species

Human
Source

HEK293
Tag

C-Flag
Accession

P08183-1 (D2-Q1280)
Gene ID

5243
Protein Length

Full Length of Isoform-1 Mature Protein
Synonyms
MDR1; PGY1
Molecular Weight

Approximately 142.5 kDa
Glycosylation
Yes
Purity

Greater than 85% as determined by reducing SDS-PAGE.
Appearance

Solution
Formulation

Supplied as a 0.22 μm filtered solution of 50 mM HEPES pH 7.5, 150 mM NaCl, 0.02% DDM, 0.002% CHS,0.25 mM TCEP, Protease inhibitor .

Endotoxin Level

<1 EU/μg, determined by LAL method.
Reconstitution

Please use rapid thawing with running water to thaw the protein.
Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping

Shipping with dry ice.
Documentation
SDS (251 KB)

COA (205 KB)

Handling Instructions (2659 KB)

ABCB1 Protein, Human (HEK293, Flag) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ABCB1 Protein, Human (HEK293, Flag)
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HY-P703175
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English - EN (251 KB)

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