(±)-NBI-74330

Cat. No.: HY-110099 Purity: 99.12%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

(±)-NBI-74330 is a potent and selective CXCR3 antagonist. (±)-NBI-74330 not only reduces tactile and thermal hypersensitivity but also enhances the analgesic properties of morphine. (±)-NBI-74330 can reduce microglial cell activation, increase astroglial cell activation, and downregulate the expression of some CXCR3 ligands in a rat neuropathic pain model.

For research use only. We do not sell to patients.

(±)-NBI-74330 Chemical Structure

CAS No. : 473722-68-8

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote	Estimated Time of Arrival: December 31
100 mg	Get quote
200 mg	Get quote

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Other Forms of (±)-NBI-74330:

NBI-74330 In-stock

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View All CXCR Isoform Specific Products:

View All Isoforms

CXCR CXCR1 CXCR2 CXCR3 CXCR4 CXCR7 CXCR6

Biological Activity
Purity & Documentation
References
Customer Review

Description

Molecular Weight

605.58

Formula

C₃₂H₂₇F₄N₅O₃

CAS No.

473722-68-8

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C2=C(N=C(C(C)N(CC3=CC=CN=C3)C(CC4=CC=C(C(C(F)(F)F)=C4)F)=O)N1C5=CC=C(C=C5)OCC)N=CC=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (165.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6513 mL	8.2565 mL	16.5131 mL
5 mM	0.3303 mL	1.6513 mL	3.3026 mL
10 mM	0.1651 mL	0.8257 mL	1.6513 mL

View the Complete Stock Solution Preparation Table

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.13 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.12%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (255 KB) HNMR (346 KB) LCMS (105 KB)

Handling Instructions (2659 KB)

References

[1]. Piotrowska A, et al. Pharmacological blockade of CXCR3 by (±)-NBI-74330 reduces neuropathic pain and enhances opioid effectiveness - Evidence from in vivo and in vitro studies. Biochim Biophys Acta Mol Basis Dis. 2018 Oct;1864(10):3418-3437. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.6513 mL	8.2565 mL	16.5131 mL	41.2827 mL
	5 mM	0.3303 mL	1.6513 mL	3.3026 mL	8.2565 mL
	10 mM	0.1651 mL	0.8257 mL	1.6513 mL	4.1283 mL
	15 mM	0.1101 mL	0.5504 mL	1.1009 mL	2.7522 mL
	20 mM	0.0826 mL	0.4128 mL	0.8257 mL	2.0641 mL
	25 mM	0.0661 mL	0.3303 mL	0.6605 mL	1.6513 mL
	30 mM	0.0550 mL	0.2752 mL	0.5504 mL	1.3761 mL
	40 mM	0.0413 mL	0.2064 mL	0.4128 mL	1.0321 mL
	50 mM	0.0330 mL	0.1651 mL	0.3303 mL	0.8257 mL
	60 mM	0.0275 mL	0.1376 mL	0.2752 mL	0.6880 mL
	80 mM	0.0206 mL	0.1032 mL	0.2064 mL	0.5160 mL
	100 mM	0.0165 mL	0.0826 mL	0.1651 mL	0.4128 mL

(±)-NBI-74330 Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(±)-NBI-74330473722-68-8CXCRCXC chemokine receptorsC-X-C motif chemokine receptorsCXCR3 antagonistthermal hypersensitivitytactileanalgesic propertiesmicroglial cellastroglial cellCXCR3 ligandsInhibitorinhibitorinhibit

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