Pyrrothiogatain

Name: Pyrrothiogatain
Brand: MedChemExpress
SKU: HY-138113
Rating: 4.5 (1 reviews)

Cat. No.: HY-138113 Purity: 98.61%: Data Sheet
COA

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Pyrrothiogatain is a transcription factor GATA3 inhibitor with an IC₅₀ of 54.7 μM. Pyrrothiogatain inhibits the DNA-binding activity of GATA3 and inhibits the T helper 2 (Th2) cell differentiation and expression of Th2 cytokines. Pyrrothiogatain shows anti-infection effect by inhibiting ACE2 expression. Pyrrothiogatain can be used for the researches of inflammation, immunology, infection and cancer, such as colon cancer and SARS-CoV-2 infection.

For research use only. We do not sell to patients.

Pyrrothiogatain Chemical Structure

CAS No. : 477888-48-5

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]^[2]^[3]^[4]

GATA3

54.7 μM (IC₅₀)

IL-4

IL-5

IL-6

IL-13

ACE

In Vitro

Pyrrothiogatain (50-100 μM) inhibits the DNA-binding activity of GATA3^[1].
Pyrrothiogatain (0-100 μM, 3 days) does not influence cell viability in Jurkat cells^[1].
Pyrrothiogatain (10-30 μM) suppresses GATA3-dependent transcriptional activation of the IL-5 promoter in HEK293T cells^[1].
Pyrrothiogatain (30-80 μM, 5 days) inhibits Th2 cell differentiation in CD4⁺ T cells^[1].
Pyrrothiogatain (80 μM, 2 days) inhibits GATA3 binding to Th2 cytokine gene locus in CD4⁺ T cells^[1].
Pyrrothiogatain (50 μM, 4 days) decreases cell ount in both NT and GATA-3 knockout 3T3L1 cells^[2].
Pyrrothiogatain (50 μM) reduces IL-6 and TNF-α levels in 3T3L-1 preadipocytes^[2].
Pyrrothiogatain (50-500 μM) reduces ACE2 and in HEK293T cells^[3].
Pyrrothiogatain (500 μM, 48 h) reduces SARS-CoV-2 viral load in Calu-3 cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Differentiation Assay^[1]

Cell Line:	CD4⁺ T cells
Concentration:	30 and 80 μM
Incubation Time:	5 days
Result:	Inhibited generation of IL-4-, IL-5-, and IL-13-producing Th2 cells. Decreased production and mRNA expression of IL-4, IL-5 and IL-13.

In Vivo

Pyrrothiogatain (60 mg/kg, i.p., once a day) inhibits MC38 tumor growth in Cdc42^+/+Foxp3^YFP-Cre mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Cdc42^+/+Foxp3^YFP-Cre mice with MC38 tumor^[4]
Dosage:	60 mg/kg
Administration:	Intraperitoneally injection, once a day
Result:	Reduced tumor volume.

Molecular Weight

221.28

Formula

C₁₁H₁₁NO₂S

CAS No.

477888-48-5

Appearance

Solid

Color

Light yellow to orange

SMILES

O=C(C1=C(N2C(C)=CC=C2C)C=CS1)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (564.90 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.5192 mL	22.5958 mL	45.1916 mL
5 mM	0.9038 mL	4.5192 mL	9.0383 mL
10 mM	0.4519 mL	2.2596 mL	4.5192 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

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Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.61%

Select Batch:

Data Sheet (283 KB) SDS (393 KB)

COA (259 KB) HNMR (237 KB) LCMS (97 KB)

Handling Instructions (2659 KB)

References

[1]. Nomura S, et al. Pyrrothiogatain acts as an inhibitor of GATA family proteins and inhibits Th2 cell differentiation in vitro. Sci Rep. 2019 Nov 22;9(1):17335. [Content Brief]

[2]. Almuraikhy S, et al. Targeted Inhibition of GATA-3 by Pyrrothiogatain: Implications for Adipocyte Biology and Inflammatory Response. Cells. 2025 Jan 10;14(2):100. [Content Brief]

[3]. Israeli M, et al. Genome-wide CRISPR screens identify GATA6 as a proviral host factor for SARS-CoV-2 via modulation of ACE2. Nat Commun. 2022 Apr 25;13(1):2237. [Content Brief]

[4]. Kalim KW, et al. Targeting of Cdc42 GTPase in regulatory T cells unleashes antitumor T-cell immunity. J Immunother Cancer. 2022 Nov;10(11):e004806. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.5192 mL	22.5958 mL	45.1916 mL	112.9790 mL
	5 mM	0.9038 mL	4.5192 mL	9.0383 mL	22.5958 mL
	10 mM	0.4519 mL	2.2596 mL	4.5192 mL	11.2979 mL
	15 mM	0.3013 mL	1.5064 mL	3.0128 mL	7.5319 mL
	20 mM	0.2260 mL	1.1298 mL	2.2596 mL	5.6490 mL
	25 mM	0.1808 mL	0.9038 mL	1.8077 mL	4.5192 mL
	30 mM	0.1506 mL	0.7532 mL	1.5064 mL	3.7660 mL
	40 mM	0.1130 mL	0.5649 mL	1.1298 mL	2.8245 mL
	50 mM	0.0904 mL	0.4519 mL	0.9038 mL	2.2596 mL
	60 mM	0.0753 mL	0.3766 mL	0.7532 mL	1.8830 mL
	80 mM	0.0565 mL	0.2824 mL	0.5649 mL	1.4122 mL
	100 mM	0.0452 mL	0.2260 mL	0.4519 mL	1.1298 mL