Polyporenic acid C

Name: Polyporenic acid C
Brand: MedChemExpress
SKU: HY-N2993
Rating: 4.5 (1 reviews)

Cat. No.: HY-N2993 Purity: 99.80%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Polyporenic acid C is a lanostane-type triterpenoid. Polyporenic acid C can be isolated from Poria cocos. Polyporenic acid C causes the cleavage of caspase-8 and caspase-3, as well as the cleavage of PARP. Polyporenic acid C reduces the phosphorylation level of Akt (Ser473), increases the phosphorylation of PTEN and p53 (Ser15), and activates JNK. Polyporenic acid C induces Apoptosis. Polyporenic acid C shows anticancer activity against non-small cell lung cancer.

For research use only. We do not sell to patients.

Polyporenic acid C Chemical Structure

CAS No. : 465-18-9

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	Get quote
200 mg	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Polyporenic acid C:

Polyporenic acid C (Standard) Get quote

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Caspase Isoform Specific Products:

View All Isoforms

Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

View All PARP Isoform Specific Products:

View All Isoforms

PARP PARP1 PARP2 PARP3 PARP4 TNKS1/PARP5A TNKS2/PARP5B PARP7 PARP10 PARP14 PARP15 PARP12 PARP16

View All Akt Isoform Specific Products:

View All Isoforms

Akt Akt1 Akt2 Akt3

View All JNK Isoform Specific Products:

View All Isoforms

JNK1 JNK2 JNK3 JNK

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

Caspase-8

Caspase-3

Cellular Effect

Cell Line	Type	Value	Description	References
BMDM	IC₅₀	4.9 μM Compound: 3	Inhibition of RANKL-induced C57BL/6 mouse BMDM differentiation into osteoclasts after 4 days in presence of M-CSF by TRAP assay Inhibition of RANKL-induced C57BL/6 mouse BMDM differentiation into osteoclasts after 4 days in presence of M-CSF by TRAP assay	[PMID: 27266877]
CCRF-CEM	IC₅₀	25.5 μM Compound: 8	Antiproliferative activity against human CCRF-CEM cells after 72 hrs by MTT assay Antiproliferative activity against human CCRF-CEM cells after 72 hrs by MTT assay	[PMID: 27808511]
HL-60	IC₅₀	31.8 μM Compound: 8	Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay	[PMID: 27808511]
K562	IC₅₀	26.3 μM Compound: 8	Antiproliferative activity against human K562 cells after 72 hrs by MTT assay Antiproliferative activity against human K562 cells after 72 hrs by MTT assay	[PMID: 27808511]
MOLT-4	IC₅₀	27.6 μM Compound: 8	Antiproliferative activity against human MOLT4 cells after 72 hrs by MTT assay Antiproliferative activity against human MOLT4 cells after 72 hrs by MTT assay	[PMID: 27808511]

In Vitro

Polyporenic acid C (0-200 μM; 24-72 h) inhibits the proliferation of human non-small cell lung cancer A549 cells in a dose- and time-dependent manner^[1].
Polyporenic acid C (60-100 μM; 24-48 h) increases the apoptosis rate of human non-small cell lung cancer A549 cells in a dose- and time-dependent manner^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	Human non-small cell lung cancer A549 cells
Concentration:	2 μM, 6 μM, 20 μM, 60 μM, 200 μM
Incubation Time:	24 h, 48 h, 72 h
Result:	Significantly inhibited cell viability (84% of the control group remained viable) (72 h, 6 μM). Reduced the relative cell viability by more than 50% (72 h, 60 μM). Inhibited cancer cell growth by more than 90% (72 h, 200 μM).

Western Blot Analysis^[1]

Cell Line:	Human non-small cell lung cancer A549 cells
Concentration:	60 μM
Incubation Time:	24 h (for caspase, Akt, PTEN, p53 detection); 3 h, 6 h, 24 h (for JNK detection)
Result:	Led to the cleavage of caspase-8 and caspase-3, and the cleavage of PARP (a substrate of caspase-3), but did not induce the cleavage of caspase-9. Reduced the phosphorylation level of Akt (Ser473), increased the phosphorylation of PTEN and p53 (Ser15), and activated JNK.

Molecular Weight

482.69

Formula

C₃₁H₄₆O₄

CAS No.

465-18-9

Appearance

Solid

Color

White to off-white

SMILES

C[C@]12[C@@]([C@]([C@H](C(O)=O)CCC(C(C)C)=C)([H])[C@H](O)C1)(CC=C3C2=CC[C@](C4(C)C)([H])[C@@]3(CCC4=O)C)C

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 16.67 mg/mL (34.54 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0717 mL	10.3586 mL	20.7172 mL
5 mM	0.4143 mL	2.0717 mL	4.1434 mL
10 mM	0.2072 mL	1.0359 mL	2.0717 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 5 mg/mL (10.36 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.80%

Select Batch:

Data Sheet (275 KB) SDS (251 KB)

COA (251 KB) HNMR (380 KB) CNMR (335 KB) RP-HPLC (253 KB) MS (169 KB)

Handling Instructions (2659 KB)

References

[1]. Ling H, et al. Polyporenic acid C induces caspase-8-mediated apoptosis in human lung cancer A549 cells.Mol Carcinog. 2009 Jun;48(6):498-507. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0717 mL	10.3586 mL	20.7172 mL	51.7931 mL
	5 mM	0.4143 mL	2.0717 mL	4.1434 mL	10.3586 mL
	10 mM	0.2072 mL	1.0359 mL	2.0717 mL	5.1793 mL
	15 mM	0.1381 mL	0.6906 mL	1.3811 mL	3.4529 mL
	20 mM	0.1036 mL	0.5179 mL	1.0359 mL	2.5897 mL
	25 mM	0.0829 mL	0.4143 mL	0.8287 mL	2.0717 mL
	30 mM	0.0691 mL	0.3453 mL	0.6906 mL	1.7264 mL