2. Anti-infection Apoptosis Metabolic Enzyme/Protease Immunology/Inflammation NF-κB Stem Cell/Wnt MAPK/ERK Pathway
  3. Bacterial Apoptosis Reactive Oxygen Species (ROS) ERK JNK Caspase SOD Fungal
  4. Ombuoside

Ombuoside has antioxidant properties, inhibiting ROS production and apoptosis. Ombuoside exerts neuroprotective effects through the ERK-JNK-caspase-3 system. Ombuoside promotes Dopamine biosynthesis through TH and CREB activation. Ombuoside exhibits antimicrobial activity against several Gram-positive and Gram-negative bacteria, as well as Candida albicans

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Ombuoside

Ombuoside Chemical Structure

CAS No. : 20188-85-6

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Based on 1 publication(s) in Google Scholar

Ombuoside Related Antibodies

Top Publications Citing Use of Products

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ERK ERK1 ERK2 ERK5 ERK8

View All JNK Isoform Specific Products:

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JNK1 JNK2 JNK3 JNK

View All Caspase Isoform Specific Products:

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Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

  • Biological Activity

  • Purity & Documentation

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Description

Ombuoside has antioxidant properties, inhibiting ROS production and apoptosis. Ombuoside exerts neuroprotective effects through the ERK-JNK-caspase-3 system. Ombuoside promotes Dopamine biosynthesis through TH and CREB activation. Ombuoside exhibits antimicrobial activity against several Gram-positive and Gram-negative bacteria, as well as Candida albicans[1][2][3][4][5]

IC50 & Target[2]

JNK1

 

ERK1

 

ERK2

 

Caspase 3

 

In Vitro

Ombuoside (0-100 μM, 24-26 h) inhibits HEI-OC1 cells growth only treatment with 80 or 100 μM, increases the survival of Cisplatin (HY-17394)-treated HEI-OC1 cells, with the 30 μM dose demonstrating the most protective effect[1].
Ombuoside (30 μM, 24 h) reduces Cisplatin-induced apoptosis, oxidative stress via the mitochondria-related apoptotic pathway in HEI-OC1 cells[1].
Ombuoside (30 μM, 24 h) protects cochlear explants from Cisplatin-induced hair cell damage by inhibiting apoptosis[1].
Ombuoside (1-100 μM, 1-72 h) reduces PC12 cells viability to 80.4% of the control level at 100 µM, increases the cell viability in L-DOPA (HY-N0304)- induced PC12 cells[2].
Ombuoside (1-10 μM, 0-6 h) decreases phosphorylation of ERK1/2 and JNK1/2, the expression of cleaved-caspase-3, increases SOD activity in L-DOPA- induced PC12 cells[2].
Ombuoside (1-10 μM, 0-24 h) increases Dopamine (HY-B0451) levels in a time- and dose-dependent manner, increases the phosphorylation of TH (Ser40) and CREB (Ser133) level in PC12 cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ombuoside Related Antibodies

Apoptosis Analysis[1]

Cell Line: HEI-OC1 cells
Concentration: 30 μM + Cisplatin (30 μM)
Incubation Time: 24 h
Result: Reduced Cisplatin-induced death and apoptosis.
Significantly decreased the expression of Bax, Bak, cytochrome c, caspase-3, increased the expression of Bcl2.

Immunofluorescence[1]

Cell Line: HEI-OC1 cells
Concentration: 30 μM + Cisplatin (30 μMl)
Incubation Time: 24 h
Result: Reduced CellROX green-positive cells and MitoSOX red-positive cells, reduced ROS levels.
Increased MitoTracker Red fluorescent signal, alleviates mitochondrial damage.

Western Blot Analysis[2][3]

Cell Line: HEI-OC1 cells
Concentration: 1, 5, and 10 µM
Incubation Time: 0, 0.5, 1, 3, 6 h
Result: Increased phosphorylated ERK1/2 at 0.5 and 1 h and then returned to the control level at 3 h.
Decreased phosphorylation of ERK1/2 by cotreatments with 1, 5, and 10 µM to 1.28-, 1.17-, and 1.08-folds of the control level.
Decreased phosphorylation of JNK1/2 by cotreatments with 1, 5, and 10 µMto 1.28-, 1.15-, and 1.06-folds of the control level.
Reduced the expression of cleaved-caspase-3 at 1, 5, and 10 µM to 1.20-, 1.09-, and 1.02-folds of the control level.
Increased SOD activity at 1, 5, and 10 µM to 80.0%, 88.0%, and 90.8% of the control level.
Significantly increased the phosphorylation of TH (Ser40) and CREB (Ser133) level.
In Vivo

Ombuoside (30 μM, 24 h) protects zebrafish hair cells from Cisplatin-induced damage[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Zebrafish model[1]
Dosage: 30 μM + Cisplatin (30 μM)
Administration: 24 h
Result: Showed a protective effect on the hair cells of zebrafish neuromasts, resulting in a higher number of hair cells and more regularly shaped cells, compared with those in the Cisplatin group.
Decreased the expression of noxal, baxb, and tp53, upregulated bcl2a.
Molecular Weight

638.57

Formula

C29H34O16
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

O=C1C(O[C@@H]([C@@H]([C@H]2O)O)O[C@@H]([C@H]2O)CO[C@H](O[C@H]3C)[C@@H]([C@@H]([C@H]3O)O)O)=C(C(C=C4)=CC(O)=C4OC)OC5=CC(OC)=CC(O)=C15
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (78.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5660 mL 7.8300 mL 15.6600 mL
5 mM 0.3132 mL 1.5660 mL 3.1320 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1.25 mg/mL (1.96 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.68%

SDS (393 KB)

COA (262 KB) HNMR (299 KB) LCMS (95 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.5660 mL 7.8300 mL 15.6600 mL 39.1500 mL
5 mM 0.3132 mL 1.5660 mL 3.1320 mL 7.8300 mL
10 mM 0.1566 mL 0.7830 mL 1.5660 mL 3.9150 mL
15 mM 0.1044 mL 0.5220 mL 1.0440 mL 2.6100 mL
20 mM 0.0783 mL 0.3915 mL 0.7830 mL 1.9575 mL
25 mM 0.0626 mL 0.3132 mL 0.6264 mL 1.5660 mL
30 mM 0.0522 mL 0.2610 mL 0.5220 mL 1.3050 mL
40 mM 0.0391 mL 0.1957 mL 0.3915 mL 0.9787 mL
50 mM 0.0313 mL 0.1566 mL 0.3132 mL 0.7830 mL
60 mM 0.0261 mL 0.1305 mL 0.2610 mL 0.6525 mL
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Ombuoside Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ombuoside20188-85-6BacterialApoptosisReactive Oxygen Species (ROS)ERKJNKCaspaseSODFungalExtracellular signal regulated kinasesc-Jun N-terminal kinaseSuperoxide DismutasePC12 cellsHEI-OC1 cellscochlear explantsZebrafishInhibitorinhibitorinhibit

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