Oil Red O Staining Kit (For Cultured Cell) 

Oil Red O is a fat-soluble diazol dye, with a maximum absorption at 518 nm. Oil Red O stains neutral lipids and cholesteryl esters but not biological membranes^[1]. Oil Red O Staining Kit (For Cultured Cell) is a staining solution specifically designed for cultured cells.

Guidelines (The following are our recommended protocols. This protocol is for reference only and should be modified according to your specific needs.)
1. Fix cultured cell samples in Cell ORO Fixative for 15-25 min.
2. Rinse with PBS buffer or physiological saline and air dry.
3. Wash with 60% isopropanol for 20-30 s.
4. Stain with oil red O staining solution for 10-15 min.
5. Rinse briefly with 60% isopropanol to remove staining solution, then rinse briefly with PBS buffer or physiological saline.
6. Counterstain cell nuclei with hematoxylin staining solution for 2-5 min.
7. Wash with ORO buffer for 1 min (optional).
8. Wash with distilled water for 8-10 min.
9. Air dry and examine under a microscope.

Staining results:
Neutral fat appears orange-red or orange-red;
Cell nuclei appear blue.

Note:
1. ORO staining solution must be prepared fresh before use.
2. Staining results cannot be stored for long periods; observe and photograph as soon as possible.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

[Oil Red O Staining Kit (For Cultured Cell)]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Escorcia W, et al. Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O Staining. J Vis Exp. 2018 Mar 5;(133):57352.  [Content Brief]

  [2]. Mehlem A, et al. Imaging of neutral lipids by oil red O for analyzing the metabolic status in health and disease. Nat Protoc. 2013 Jun;8(6):1149-54.  [Content Brief]