ODN 1826  (Synonyms: CpG 1826)

ODN 1826 is a class B CpG ODN (oligodeoxynucleotide) and TLR9 agonist. ODN 1826 induces NO and iNOS production and enhances Apoptosis. ODN 1826 enhances immune surveillance. ODN 1826 increases aortic atherosclerotic plaque size. ODN 1826 has antitumor activity against lung cancer, glioma and melanoma^{[1][2][3][4][5][6]}.

ODN 1826 (1 μg/mL; 24 h) induces nitric oxide (NO) and inducible nitric oxide synthase (iNOS) production in RAW 264.7 mouse macrophages^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CpG-1826 (100 μg/tumor; intratumoral injection; every 3 days for up to 4 times) delays the growth of subcutaneously implanted GL261 gliomas in C57BL/6NTac mice, inducing increased infiltration of macrophages and B cells into tumors, with significant growth delay in ~50% of mice^[4]. ODN 1826 (18 nM; s.c.; 3 times a week for 7 weeks) increases aortic atherosclerotic plaque size in a chronic vascular injury mouse model^[5].
ODN 1826 (0.05 mg; i.p.; 1, 3, 5, 8, 11, 13 days) has a strong anti-tumor growth effect in a Lewis lung cancer mouse tumor model^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

6364.10

202668-42-6

Solid

White to off-white

DNA, d(P-thio)(T-C-C-A-T-G-A-C-G-T-T-C-C-T-G-A-C-G-T-T)

[ODN 1826]

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Hou Y, et al. Lipoamide Ameliorates Oxidative Stress via Induction of Nrf2/ARE Signaling Pathway in PC12 Cells. J Agric Food Chem. 2019 Jul 24;67(29):8227-8234.  [Content Brief]

  [2]. Qi XF, et al. CpG oligodeoxynucleotide induces apoptosis and cell cycle arrest in A20 lymphoma cells via TLR9-mediated pathways. Mol Immunol. 2013 Jul;54(3-4):327-37.  [Content Brief]

  [3]. Utaisincharoen P, et al. CpG ODN activates NO and iNOS production in mouse macrophage cell line (RAW 264.7). Clin Exp Immunol. 2002 Jun;128(3):467-73.  [Content Brief]

  [4]. Jordan M, et al. CpG-1826 immunotherapy potentiates chemotherapeutic and anti-tumor immune responses to metronomic cyclophosphamide in a preclinical glioma model. Cancer Lett. 2016 Apr 1;373(1):88-96.  [Content Brief]

  [5]. Krogmann AO, et al. Proinflammatory Stimulation of Toll-Like Receptor 9 with High Dose CpG ODN 1826 Impairs Endothelial Regeneration and Promotes Atherosclerosis in Mice. PLoS One. 2016 Jan 11;11(1):e0146326.  [Content Brief]

  [6]. Yuan S, et al. CpG oligodeoxynucleotide 1826 enhances the Lewis lung cancer response to radiotherapy in murine tumor. Cancer Biother Radiopharm. 2011 Apr;26(2):203-8.  [Content Brief]