Nicotinamide Hydrochloride (Synonyms: Niacinamide Hydrochloride; Nicotinic acid amide Hydrochloride)

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Nicotinamide Hydrochloride is a form of vitamin B3 or niacin. Nicotinamide Hydrochloride inhibits SIRT2 activity (IC₅₀: 2 μM). Nicotinamide Hydrochloride also inhibits SIRT1. Nicotinamide Hydrochloride increases cellular NAD₊, ATP, ROS levels. Nicotinamide Hydrochloride inhibits tumor growth and improves survival. Nicotinamide Hydrochloride also has anti-HBV activity.

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Nicotinamide Hydrochloride Chemical Structure

CAS No. : 25334-23-0

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Other In-stock Forms of Nicotinamide Hydrochloride:

Nicotinamide

Other Forms of Nicotinamide Hydrochloride:

Nicotinamide In-stock

58 Publications Citing Use of MCE Nicotinamide Hydrochloride

: Nicotinamide Hydrochloride purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2024 Dec 31:107573. [Abstract]; Nicotinamide (NAM) (10 μM) enhanced ISO (10 μM)-induced YAP acetylation in neonatal rat cardiomyocytes (NRCMs).

: Nicotinamide Hydrochloride purchased from MedChemExpress. Usage Cited in: Oncoimmunology. 2025 Dec;14(1):2460281. [Abstract]; Nicotinamide (NAM) (6 h) promotes CPT2 acetylation in SK-N-AS cells.

: Nicotinamide Hydrochloride purchased from MedChemExpress. Usage Cited in: Apoptosis. 2024 Dec 25. [Abstract]; Nicotinamide (NAM) (20 mM; pretreatment for 24 hours before LPS exposure in MLE-12 cells) increases GPX4 acetylation levels in MLE-12 cells.

: Nicotinamide Hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2023 Oct 12. [Abstract]; Nicotinamide (NAM) (10 mM, 4 h) enhances RACK1 acetylation levels in OVCAR3 and SKOV3 cells in combination with Trichostatin A (TSA) (HY-15144) (0.2 μM, 4 h).

: Nicotinamide Hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2022 May 17;8(1):265. [Abstract]; Nicotinamide (NAM) (6-8 h) upregulates stathmin1 acetylation in 293T cells transfected with stathmin1-Flag plasmids.

: Nicotinamide Hydrochloride purchased from MedChemExpress. Usage Cited in: Nat Commun. 2021 Jul 9;12(1):4227. [Abstract]; Nicotinamide (80 μM; 4-8 h) markedly increases GLDC K514 acetylation in U251 and U87 cells.

: Nicotinamide Hydrochloride purchased from MedChemExpress. Usage Cited in: Hum Reprod. 2020 Mar 27;35(3):494-503. [Abstract]; Nicotinamide (NAM) (40 mM; 1 h) increases the global level of lysine 2-hydroxyisobutyrylation along the human sperm tail.

: Nicotinamide Hydrochloride purchased from MedChemExpress. Usage Cited in: Hum Reprod. 2019 Jul 8;34(7):1186-1194. [Abstract]; Nicotinamide (NAM) (20-40 mM; 1 h) increases global levels of Kac (lysine acetylation) and Kglu (lysine glutarylation) in human sperm.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]^[2]

Human Endogenous Metabolite

SIRT2

2 μM (EC₅₀)

SIRT1

50-180 μM (IC₅₀)

In Vitro

Nicotinamide Hydrochloride (0-50 mM, 24/48 h ) reduces cell number in a time-dependent and dose-dependent manner in A375 and SK-MEL-28 cells^[1].
Nicotinamide Hydrochloride (10-50 mM, 24 h ) makes A375 cells undergo accumulation in G1 phase, reduction in S phase, and increase inthe sub-G1 (apoptosis) phase^[1].
Nicotinamide Hydrochloride (1-50 mM, 6 h ) increases NAD₊, ATP and ROS levels in A375 and SK-MEL-28 cells^[1].
Nicotinamide Hydrochloride (0.01-20 mM, 1 h) inhibits purified SIRT2 enzymatic activity in vitro with an EC₅₀ of 2 μM^[1].
Nicotinamide Hydrochloride (0-64 mM) inhibits HBV replication in HepAD38 and HepG2.2.15 cells^[3].
Nicotinamide Hydrochloride (10 mM, on day 13) promotes pancreatic cell differentiation from human embryonic stem cells (hESCs) through CK1 and ROCK inhibition^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	A375, SK-MEL-28, mouse B16-F10 cell
Concentration:	0, 1, 20, 50 mM
Incubation Time:	24 h, 48 h
Result:	Reduced cell number in a dose-dependent manner with a strong inhibitory effect at 20mM and an almost complete effect at 50 mM.

Cell Cycle Analysis^[1]

Cell Line:	A375, SK-MEL-28
Concentration:	10, 20, 50 mM
Incubation Time:	24 h
Result:	Arrested A375 cells in G1 phase.

In Vivo

Nicotinamide Hydrochloride (Intraperitoneal injection, 1500 and 1800 mg/Kg, 5 days per week) inhibits tumor growth in murine metastatic melanoma model^[1].
Nicotinamide Hydrochloride (Intraperitoneal injection, 1800 mg/Kg, once a day, murine metastatic melanoma model) affects IFN-γ (a key mediator of cell-mediated anti-tumor immunity), increases the plasma levels of Eotaxin and IL-5, reduces the plasma levels of IL-3, IL-12, RANTES and IL-10^[1].
Nicotinamide Hydrochloride (vein injection, 0-200 mg/kg, 5 days) inhibits HBV replication in HBV-transgenic mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6 mice (subcutaneous injected with B16-F10 cells)^[1]
Dosage:	1000, 1500, 1800 mg/Kg.
Administration:	Intraperitoneal injection, 5 days per week (followed by 2-day rest) or once a day.
Result:	Inhibited tumor growth at 1500 and 1800 mg/Kg, and had no effect on the body weight. Increased the frequency of IFN-γ producing cells and modulated the protein levels of cytokines and chemokines in the plasma of tumor-bearing mice.

Animal Model:	HBV-transgenic mice^[3]
Dosage:	0-200 mg/kg
Administration:	Vein injection, 5 days
Result:	Reduced serum HBV DNA.

Clinical Trial

Molecular Weight

158.59

Formula

C₆H₇ClN₂O

CAS No.

25334-23-0

SMILES

O=C(C1=CC=CN=C1)N.[H]Cl

Structure Classification

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Francesca Scatozza, et al. Nicotinamide inhibits melanoma in vitro and in vivo. J Exp Clin Cancer Res. 2020 Oct 7;39(1):211. [Content Brief]

[2]. Hwang ES, et al. Nicotinamide is an inhibitor of SIRT1 in vitro, but can be a stimulator in cells. Cell Mol Life Sci. 2017 Sep;74(18):3347-3362. [Content Brief]

[3]. Li WY, et al. The SIRT1 inhibitor, nicotinamide, inhibits hepatitis B virus replication in vitro and in vivo. Arch Virol. 2016 Mar;161(3):621-30. [Content Brief]

[4]. Zhang Y, et al. Nicotinamide promotes pancreatic differentiation through the dual inhibition of CK1 and ROCK kinases in human embryonic stem cells. Stem Cell Res Ther. 2021 Jun 25;12(1):362. [Content Brief]

Nicotinamide Hydrochloride Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Nicotinamide25334-23-0NiacinamideNicotinic acid amideSirtuinEndogenous MetaboliteHBVHepatitis B virusMelanomatumorA375 cellSK-MEL-28 cellskin cancerhESCsInhibitorinhibitorinhibit

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