Nicotinamide (Synonyms: Niacinamide; Nicotinic acid amide)

Name: Nicotinamide
Brand: MedChemExpress
SKU: HY-B0150
Rating: 5.0 (66 reviews)

Cat. No.: HY-B0150 Purity: 99.99%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

Nicotinamide is a form of vitamin B3 or niacin. Nicotinamide Hydrochloride inhibits SIRT2 activity (IC₅₀: 2 μM). Nicotinamide also inhibits SIRT1. Nicotinamide increases cellular NAD₊, ATP, ROS levels. Nicotinamide inhibits tumor growth and improves survival. Nicotinamide also has anti-HBV activity.

For research use only. We do not sell to patients.

Nicotinamide Chemical Structure

CAS No. : 98-92-0

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Based on 66 publication(s) in Google Scholar

Other Forms of Nicotinamide:

58 Publications Citing Use of MCE Nicotinamide

: Nicotinamide purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2024 Dec 31:107573. [Abstract]; Nicotinamide (NAM) (10 μM) enhanced ISO (10 μM)-induced YAP acetylation in neonatal rat cardiomyocytes (NRCMs).

: Nicotinamide purchased from MedChemExpress. Usage Cited in: Oncoimmunology. 2025 Dec;14(1):2460281. [Abstract]; Nicotinamide (NAM) (6 h) promotes CPT2 acetylation in SK-N-AS cells.

: Nicotinamide purchased from MedChemExpress. Usage Cited in: Apoptosis. 2024 Dec 25. [Abstract]; Nicotinamide (NAM) (20 mM; pretreatment for 24 hours before LPS exposure in MLE-12 cells) increases GPX4 acetylation levels in MLE-12 cells.

: Nicotinamide purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2023 Oct 12. [Abstract]; Nicotinamide (NAM) (10 mM, 4 h) enhances RACK1 acetylation levels in OVCAR3 and SKOV3 cells in combination with Trichostatin A (TSA) (HY-15144) (0.2 μM, 4 h).

: Nicotinamide purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2022 May 17;8(1):265. [Abstract]; Nicotinamide (NAM) (6-8 h) upregulates stathmin1 acetylation in 293T cells transfected with stathmin1-Flag plasmids.

: Nicotinamide purchased from MedChemExpress. Usage Cited in: Nat Commun. 2021 Jul 9;12(1):4227. [Abstract]; Nicotinamide (80 μM; 4-8 h) markedly increases GLDC K514 acetylation in U251 and U87 cells.

: Nicotinamide purchased from MedChemExpress. Usage Cited in: Hum Reprod. 2020 Mar 27;35(3):494-503. [Abstract]; Nicotinamide (NAM) (40 mM; 1 h) increases the global level of lysine 2-hydroxyisobutyrylation along the human sperm tail.

: Nicotinamide purchased from MedChemExpress. Usage Cited in: Hum Reprod. 2019 Jul 8;34(7):1186-1194. [Abstract]; Nicotinamide (NAM) (20-40 mM; 1 h) increases global levels of Kac (lysine acetylation) and Kglu (lysine glutarylation) in human sperm.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]^[2]

Human Endogenous Metabolite

SIRT2

2 μM (EC₅₀)

SIRT1

50-180 μM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
RAW264.7	IC₅₀	> 10 μM Compound: 17	Antiinflammatory activity in mouse RAW264.7 cells assessed as reduction in LPS-induced NO production pretreated for 30 mins followed by LPS stimulation for 24 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as reduction in LPS-induced NO production pretreated for 30 mins followed by LPS stimulation for 24 hrs by Griess assay	[PMID: 28499733]

In Vitro

Nicotinamide (0-50 mM, 24/48 h ) reduces cell number in a time-dependent and dose-dependent manner in A375 and SK-MEL-28 cells^[1].
Nicotinamide (10-50 mM, 24 h ) makes A375 cells undergo accumulation in G1 phase, reduction in S phase, and increase inthe sub-G1 (apoptosis) phase^[1].
Nicotinamide (1-50 mM, 6 h ) increases NAD₊, ATP and ROS levels in A375 and SK-MEL-28 cells^[1].
Nicotinamide Hydrochloride (0.01-20 mM, 1 h) inhibits purified SIRT2 enzymatic activity in vitro with an EC₅₀ of 2 μM^[1].
Nicotinamide (0-64 mM) inhibits HBV replication in HepAD38 and HepG2.2.15 cells^[3].
Nicotinamide (10 mM, on day 13) promotes pancreatic cell differentiation from human embryonic stem cells (hESCs) through CK1 and ROCK inhibition^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	A375, SK-MEL-28, mouse B16-F10 cell
Concentration:	0, 1, 20, 50 mM
Incubation Time:	24 h, 48 h
Result:	Reduced cell number in a dose-dependent manner with a strong inhibitory effect at 20mM and an almost complete effect at 50 mM.

Cell Cycle Analysis^[1]

Cell Line:	A375, SK-MEL-28
Concentration:	10, 20, 50 mM
Incubation Time:	24 h
Result:	Arrested A375 cells in G1 phase.

In Vivo

Nicotinamide Hydrochloride (Intraperitoneal injection, 1500 and 1800 mg/Kg, 5 days per week) inhibits tumor growth in murine metastatic melanoma model^[1].
Nicotinamide Hydrochloride (Intraperitoneal injection, 1800 mg/Kg, once a day, murine metastatic melanoma model) affects IFN-γ (a key mediator of cell-mediated anti-tumor immunity), increases the plasma levels of Eotaxin and IL-5, reduces the plasma levels of IL-3, IL-12, RANTES and IL-10^[1].
Nicotinamide Hydrochloride (vein injection, 0-200 mg/kg, 5 days) inhibits HBV replication in HBV-transgenic mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6 mice (subcutaneous injected with B16-F10 cells)^[1]
Dosage:	1000, 1500, 1800 mg/Kg.
Administration:	Intraperitoneal injection, 5 days per week (followed by 2-day rest) or once a day.
Result:	Inhibited tumor growth at 1500 and 1800 mg/Kg, and had no effect on the body weight. Increased the frequency of IFN-γ producing cells and modulated the protein levels of cytokines and chemokines in the plasma of tumor-bearing mice.

Animal Model:	HBV-transgenic mice^[3]
Dosage:	0-200 mg/kg
Administration:	Vein injection, 5 days
Result:	Reduced serum HBV DNA.

Clinical Trial

Molecular Weight

122.12

Formula

C₆H₆N₂O

CAS No.

98-92-0

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=CC=CN=C1)N

Structure Classification

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (409.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : ≥ 50 mg/mL (409.43 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	8.1887 mL	40.9433 mL	81.8867 mL
5 mM	1.6377 mL	8.1887 mL	16.3773 mL
10 mM	0.8189 mL	4.0943 mL	8.1887 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (20.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (20.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (20.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (818.87 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.99%

Select Batch:

Data Sheet (280 KB) SDS (393 KB)

COA (247 KB) RP-HPLC (276 KB) LCMS (97 KB)

Handling Instructions (2659 KB)

References

[1]. [1] Francesca Scatozza, et al. Nicotinamide inhibits melanoma in vitro and in vivo. J Exp Clin Cancer Res. 2020 Oct 7;39(1):211. [Content Brief]

[2]. Hwang ES, et al. Nicotinamide is an inhibitor of SIRT1 in vitro, but can be a stimulator in cells. Cell Mol Life Sci. 2017 Sep;74(18):3347-3362. [Content Brief]

[3]. Li WY, et al. The SIRT1 inhibitor, nicotinamide, inhibits hepatitis B virus replication in vitro and in vivo. Arch Virol. 2016 Mar;161(3):621-30. [Content Brief]

[4]. Zhang Y, et al. Nicotinamide promotes pancreatic differentiation through the dual inhibition of CK1 and ROCK kinases in human embryonic stem cells. Stem Cell Res Ther. 2021 Jun 25;12(1):362. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	8.1887 mL	40.9433 mL	81.8867 mL	204.7167 mL
	5 mM	1.6377 mL	8.1887 mL	16.3773 mL	40.9433 mL
	10 mM	0.8189 mL	4.0943 mL	8.1887 mL	20.4717 mL
	15 mM	0.5459 mL	2.7296 mL	5.4591 mL	13.6478 mL
	20 mM	0.4094 mL	2.0472 mL	4.0943 mL	10.2358 mL
	25 mM	0.3275 mL	1.6377 mL	3.2755 mL	8.1887 mL
	30 mM	0.2730 mL	1.3648 mL	2.7296 mL	6.8239 mL
	40 mM	0.2047 mL	1.0236 mL	2.0472 mL	5.1179 mL
	50 mM	0.1638 mL	0.8189 mL	1.6377 mL	4.0943 mL
	60 mM	0.1365 mL	0.6824 mL	1.3648 mL	3.4119 mL
	80 mM	0.1024 mL	0.5118 mL	1.0236 mL	2.5590 mL
	100 mM	0.0819 mL	0.4094 mL	0.8189 mL	2.0472 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Nicotinamide Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Nicotinamide98-92-0Niacinamide Nicotinic acid amideOrganoidEndogenous MetaboliteSirtuinHBVHepatitis B virusMelanomatumorA375 cellSK-MEL-28 cellskin cancerhESCsInhibitorinhibitorinhibit

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Nicotinamide (Synonyms: Niacinamide; Nicotinic acid amide)

Customer Review

Other Forms of Nicotinamide:

Nicotinamide Related Antibodies

58 Publications Citing Use of MCE Nicotinamide

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Sirtuin Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Nicotinamide Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Nicotinamide Related Classifications

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