2. Neuronal Signaling Membrane Transporter/Ion Channel Immunology/Inflammation Metabolic Enzyme/Protease Anti-infection
  3. TRP Channel COX Phosphatase Bacterial
  4. Methyl syringate

Methyl syringate is a selective TRPA1 agonist. Methyl syringate regulates food intake and gastric emptying through a TRPA1-mediated pathway. Methyl syringate is an efficient phenolic mediator for bacterial and fungal laccases. Methyl syringate is a chemical marker of Asphodel monofloral honey. Methyl syringate contributes to the antibacterial activity of honey. Methyl syringate inhibits aflatoxin production. Methyl syringate can contribute to weight suppression. Methyl syringate can be studies for cancer prevention (e.g. lung cancer), suppression of hypoxia-induced inflammatory response and tumorigenesis.

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Methyl syringate Chemical Structure

Methyl syringate Chemical Structure

CAS No. : 884-35-5

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO In-stock
Solid
500 mg In-stock
1 g In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of Methyl syringate:

Methyl syringate Related Antibodies

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Description

Methyl syringate is a selective TRPA1 agonist. Methyl syringate regulates food intake and gastric emptying through a TRPA1-mediated pathway. Methyl syringate is an efficient phenolic mediator for bacterial and fungal laccases. Methyl syringate is a chemical marker of Asphodel monofloral honey. Methyl syringate contributes to the antibacterial activity of honey. Methyl syringate inhibits aflatoxin production. Methyl syringate can contribute to weight suppression. Methyl syringate can be studies for cancer prevention (e.g. lung cancer), suppression of hypoxia-induced inflammatory response and tumorigenesis[1][2][3][4].

IC50 & Target

TRPA1[3].
In Vitro

Methyl syringate (0.1-1 mM, pretreatment for 1 h then co-treatment under hypoxia for 24 h) effectively blocks hypoxia-induced COX-2 protein expression is a dose dependent manner and inhibits induction of COX-2 mRNA and promoter activity in A549 cells under hypoxic conditions[4].
Methyl syringate (1 mM, 1 h) represses COX-2 and cell migration in HT-1080 cells[4].
Methyl syringate (1-100 μM) inhibits catalytic activity of PTPN2 and PTPN6 with IC50 values of 6.95 and 7.31 μM in E. coli cells overexpressing PTPN2 and PTPN6[5].
Methyl syringate (10-20 μM, 6 h) stimulates the glucose uptake in differentiated 3T3-L1 adipocytes and C2C12 muscle cells[5].
Methyl syringate (5-20 μM, 6 h) significantly increases AMPK phosphorylation in 3T3-L1 adipocytes at 10 or 20 μM dosage[5].
Methyl syringate (1-20 μM, 6 d) does not affect adipocyte differentiation in differentiated 3T3-L1 preadipocytes, indicating it does not induce lipid accumulation[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Methyl syringate Related Antibodies

Western Blot Analysis[4]

Cell Line: A549 under hypoxic conditions
Concentration: 1 mM
Incubation Time: 1 h
Result: Inhibited hypoxia-induced COX-2 protein through TRPA1.
Was not affected by Sirt1 enzyme on its inhibition of hypoxia-induced COX-2 protein expression.
Was blocked by HC-030031 (HY-15064) (100 μM) on its inhibition of hypoxia-induced COX-2 protein expression under hypoxic conditions.

ELISA Assay[4]

Cell Line: A549 under hypoxic conditions
Concentration: 1 mM
Incubation Time: 1 h
Result: Decreased A549 migration under hypoxic conditions by approximately 40%.
Blocked by HC-030031 (100 μM) on its inhibition of cell migration, suggesting that its effects on hypoxia-induced migration are mediated by TRPA1.
Decreased hypoxia-enhanced A549 invasion.
In Vivo

Methyl syringate (10 mg/kg, p.o., one single dose) significantly reduces the cumulative food intake 24 h after treatment in male ICR mice experienced fasting and allowed ad libitum access of food and water[1].
Methyl syringate (0.1-10 mg/kg, p.o., one single dose) significantly decreases the food remaining in the stomach 28 h after treatment in male ICR mice [1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male ICR mice experienced fasting and allowed ad libitum access of food and water[1]
Dosage: 0.1-10 mg/kg
Administration: Oral gavage (p.o.)
Result: Did not alter the body weight significantly compared to the control.
Delayed gastric emptying dose-dependently with around 70% reduction at 10 mg/kg compared to the control.
Increased plasma PYY levels 15 min after treatment but did not affect GLP-1 levels.
Molecular Weight

212.20

Formula

C10H12O5
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(OC)C1=CC(OC)=C(O)C(OC)=C1
Structure Classification
Initial Source

honey
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (471.25 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.7125 mL 23.5627 mL 47.1254 mL
5 mM 0.9425 mL 4.7125 mL 9.4251 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (11.78 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (11.78 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
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%
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.96%

SDS (393 KB)

COA (250 KB) HNMR (118 KB) RP-HPLC (281 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.7125 mL 23.5627 mL 47.1254 mL 117.8134 mL
5 mM 0.9425 mL 4.7125 mL 9.4251 mL 23.5627 mL
10 mM 0.4713 mL 2.3563 mL 4.7125 mL 11.7813 mL
15 mM 0.3142 mL 1.5708 mL 3.1417 mL 7.8542 mL
20 mM 0.2356 mL 1.1781 mL 2.3563 mL 5.8907 mL
25 mM 0.1885 mL 0.9425 mL 1.8850 mL 4.7125 mL
30 mM 0.1571 mL 0.7854 mL 1.5708 mL 3.9271 mL
40 mM 0.1178 mL 0.5891 mL 1.1781 mL 2.9453 mL
50 mM 0.0943 mL 0.4713 mL 0.9425 mL 2.3563 mL
60 mM 0.0785 mL 0.3927 mL 0.7854 mL 1.9636 mL
80 mM 0.0589 mL 0.2945 mL 0.5891 mL 1.4727 mL
100 mM 0.0471 mL 0.2356 mL 0.4713 mL 1.1781 mL
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Methyl syringate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Methyl syringate884-35-5TRP ChannelCOXPhosphataseBacterialTransient receptor potential channelsCyclooxygenaseTRPA1PYYweight suppressionfood intakephenolic mediatormonofloral honeyhypoxiaInhibitorinhibitorinhibit

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