2. Academic Validation
  3. (R)-citramalate synthase in methanogenic archaea

(R)-citramalate synthase in methanogenic archaea

  • J Bacteriol. 1999 Jan;181(1):331-3. doi: 10.1128/JB.181.1.331-333.1999.
D M Howell 1,  H Xu,  R H White
Affiliations

Affiliation

  • 1 Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061-0308, USA.
PMID: 9864346 DOI: 10.1128/JB.181.1.331-333.1999
Abstract

The Methanococcus jannaschii gene MJ1392 was cloned, and its protein product was hyperexpressed in Escherichia coli. The resulting protein was purified and shown to catalyze the condensation of pyruvate and acetyl coenzyme A, with the formation of (R)-citramalate. Thus, this gene (cimA) encodes an (R)-citramalate synthase (CimA). This is the first identification of this enzyme, which is likely involved in the biosynthesis of isoleucine.

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