2. Academic Validation
  3. Photoconverted cells allow rapid assessment of vaccine adjuvant potency in mice

Photoconverted cells allow rapid assessment of vaccine adjuvant potency in mice

  • iScience. 2025 Jun 5;28(7):112774. doi: 10.1016/j.isci.2025.112774.
Yiwei Zhong 1 2,  Mingyue Chen 1 2,  Hongzhe Lin 1,  Cheng Zu 1 2,  Yue He 1 3,  Bin Wang 1 2 3
Affiliations

Affiliations

  • 1 MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, Shanghai Institute of Infectious Disease and Biosecurity, Shanghai Frontiers Science Center of Pathogenic Microorganisms and Infection, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China.
  • 2 Shanghai Institute of Infectious Disease and Biosecurity, Fudan University, Shanghai 200032, China.
  • 3 Advaccine Biopharmaceuticals (Suzhou) Co. Ltd, Suzhou 215000, China.
PMID: 40606756 DOI: 10.1016/j.isci.2025.112774
Abstract

Identifying vaccine adjuvants that optimally enhance CD8+ T cell responses remain a challenge, often requiring time-consuming and resource-intensive methods. Here, we introduce a photoconversion-based approach using KikGR mice to track migratory dendritic cells (Mig DCs) within 48 h post-vaccination. This method enables real-time visualization of skin-derived Mig DCs migration to draining lymph nodes (dLNs), providing an early and reliable predictor of CD8+ T cell priming and anti-tumor efficacy. Unlike traditional techniques that demand extensive experimentation, this system allows for faster, cost-effective screening of adjuvants. We further demonstrate that blocking CCR7 significantly reduces Mig DCs migration, impairing CD8+ T cell responses. Our approach revolutionizes Adjuvant evaluation, enabling swift and accurate immune assessments, particularly in therapeutic vaccine development.

Keywords

Immunology; Methodology in biological sciences.

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