2. Academic Validation
  3. Separation and determination of fluindapyr enantiomers in cucumber and tomato and by supercritical fluid chromatography tandem mass spectrometry

Separation and determination of fluindapyr enantiomers in cucumber and tomato and by supercritical fluid chromatography tandem mass spectrometry

  • Food Chem. 2022 Nov 30:395:133571. doi: 10.1016/j.foodchem.2022.133571.
Peilin Guo 1,  Xiaokang An 1,  Wuying Chen 2,  Xinglu Pan 1,  Runan Li 1,  Jun Xu 1,  Xiaohu Wu 1,  Yongquan Zheng 3,  Fengshou Dong 4
Affiliations

Affiliations

  • 1 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China.
  • 2 Hunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, PR China.
  • 3 Colleage of Plant Health and Medicine, and Key Lab of Integrated Crop Disease and Pest Management of Shan-dong Province, Qingdao Agricultural University, Qingdao 266109, PR China. Electronic address: zhengyongquan@ippcaas.cn.
  • 4 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China. Electronic address: dongfengshou@caas.cn.
PMID: 35802974 DOI: 10.1016/j.foodchem.2022.133571
Abstract

Fluindapyr is a pyrazolamide chiral fungicide of Succinate Dehydrogenase Inhibitor (SDHIs) with two enantiomers. Pesticide enantiomers often exhibit different biological activities, toxicity due to their different enantioselectivity. Therefore, it is important to separate fluindapyr enantiomers and assess each enantiomer. In this study, fluindapyr enantiomers were baseline separated by supercritical fluid chromatography-mass spectrometry in 2 min. The limit of quantification (LOQ) of this method was 5 μg/kg. The developed method was applied to monitor the fluindapyr enantiomers in cucumber and tomato, the data showed that R-(-)-fluindapyr was preferentially degraded in tomato leaves, S-(+)-fluindapyr was preferentially degraded in cucumber leaves, and fluindapyr enantiomers had no enantioselective degradation behavior in two fruits. It is proved again that enantiomers have different enantioselective degradation behavior with the different plant species and even to different parts of the same plant. The enantioselectivity is likely to be caused by different biodegradation enzyme systems.

Keywords

Cucumber; Enantioselectivity; Fluindapyr; Supercritical Fluid Chromatography Tandem Mass Spectrometry (SFC-MS/MS); Tomato.

Figures
Products