2. Academic Validation
  3. Osteoprotective Effects of Loganic Acid on Osteoblastic and Osteoclastic Cells and Osteoporosis-Induced Mice

Osteoprotective Effects of Loganic Acid on Osteoblastic and Osteoclastic Cells and Osteoporosis-Induced Mice

  • Int J Mol Sci. 2020 Dec 28;22(1):233. doi: 10.3390/ijms22010233.
Eunkuk Park 1 2,  Chang Gun Lee 1 2,  Eunguk Lim 1 2,  Seokjin Hwang 1 2,  Seung Hee Yun 1 2,  Jeonghyun Kim 1 2,  Hyesoo Jeong 3,  Yoonjoong Yong 3,  Seong-Hoon Yun 3,  Chun Whan Choi 4,  Hyun-Seok Jin 5,  Seon-Yong Jeong 1 2 3
Affiliations

Affiliations

  • 1 Department of Medical Genetics, Ajou University School of Medicine, Suwon 16499, Korea.
  • 2 Department of Biomedical Sciences, Ajou University Graduate School of Medicine, Suwon 16499, Korea.
  • 3 Nine B Company, Daejeon 34121, Korea.
  • 4 Natural Products Research Institute, Gyeonggi Institute of Science & Technology Promotion, Suwon 16229, Korea.
  • 5 Department of Biomedical Laboratory Science, College of Life and Health Sciences, Hoseo University, Asan 31499, Korea.
PMID: 33379387 DOI: 10.3390/ijms22010233
Abstract

Osteoporosis is a common disease caused by an imbalance of processes between bone resorption by osteoclasts and bone formation by osteoblasts in postmenopausal women. The roots of Gentiana lutea L. (GL) are reported to have beneficial effects on various human diseases related to liver functions and gastrointestinal motility, as well as on arthritis. Here, we fractionated and isolated bioactive constituent(s) responsible for anti-osteoporotic effects of GL root extract. A single phytochemical compound, loganic acid, was identified as a candidate osteoprotective agent. Its anti-osteoporotic effects were examined in vitro and in vivo. Treatment with loganic acid significantly increased osteoblastic differentiation in preosteoblast MC3T3-E1 cells by promoting Alkaline Phosphatase activity and increasing mRNA expression levels of bone metabolic markers such as Alpl, Bglap, and Sp7. However, loganic acid inhibited osteoclast differentiation of primary-cultured monocytes derived from mouse bone marrow. For in vivo experiments, the effect of loganic acid on ovariectomized (OVX) mice was examined for 12 weeks. Loganic acid prevented OVX-induced bone mineral density loss and improved bone structural properties in osteoporotic model mice. These results suggest that loganic acid may be a potential therapeutic candidate for treatment of osteoporosis.

Keywords

bone mineral density; loganic acid; natural plant; osteoblast; osteoclast; osteoporosis; ovariectomized mice.

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