2. Academic Validation
  3. Inhibition potential of phenolic constituents from the aerial parts of Tetrastigma hemsleyanum against soluble epoxide hydrolase and nitric oxide synthase

Inhibition potential of phenolic constituents from the aerial parts of Tetrastigma hemsleyanum against soluble epoxide hydrolase and nitric oxide synthase

  • J Enzyme Inhib Med Chem. 2019 Dec;34(1):753-760. doi: 10.1080/14756366.2019.1584621.
Cai Yi Wang 1,  Sunggun Lee 1,  Hyun-Jae Jang 2,  Xiang Dong Su 1,  Heng-Shan Wang 3,  Young Ho Kim 1,  Seo Young Yang 1
Affiliations

Affiliations

  • 1 a College of Pharmacy, Chungnam National University , Daejeon , Korea.
  • 2 b Immunoregulatory Material Research Center, Korea Research Institute of Bioscience and Biotechnology , Jeongeup-si , Jeonbuk , Korea.
  • 3 c State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University , Guilin , People's Republic of China.
PMID: 30829084 DOI: 10.1080/14756366.2019.1584621
Abstract

The aerial parts of Tetrastigma hemsleyanum (APTH) have been used as a functional tea in China. The purpose of the current study was to identify the bioactive constituents with inhibitory activity against soluble Epoxide Hydrolase (sEH) and inducible nitric oxide synthase (iNOS), which are jointly considered potential therapeutic targets for vascular system diseases. In the present study, 39 compounds (1-39) were isolated from the APTH. Among them, compounds 8, 10, 12, 16, 17, 19, and 32 displayed potential activities, with IC50 values ranging from 4.5 to 9.5 µM, respectively, and all in non-competitive inhibition mode. Compounds 5, 10, 12, 19, and 32 displayed potent iNOS inhibitory effects, with IC50 values ranging from 15.6 to 47.3 µM. The results obtained in this work contribute to a better understanding of the pharmacological activities of T. hemsleyanum and its potential application as a functional food.

Keywords

nitric oxide synthase; phenolics; soluble epoxide hydrolase; vitaceae.

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