2. Academic Validation
  3. In Situ Articular Cartilage Regeneration through Endogenous Reparative Cell Homing Using a Functional Bone Marrow-Specific Scaffolding System

In Situ Articular Cartilage Regeneration through Endogenous Reparative Cell Homing Using a Functional Bone Marrow-Specific Scaffolding System

  • ACS Appl Mater Interfaces. 2018 Nov 14;10(45):38715-38728. doi: 10.1021/acsami.8b11687.
Xun Sun 1 2,  Heyong Yin 3,  Yu Wang 1,  Jiaju Lu 4,  Xuezhen Shen 1,  Changfeng Lu 1,  He Tang 1,  Haoye Meng 1,  Shuhui Yang 4,  Wen Yu 1,  Yun Zhu 5,  Quanyi Guo 1,  Aiyuan Wang 1,  Wenjing Xu 1,  Shuyun Liu 1,  Shibi Lu 1,  Xiumei Wang 4,  Jiang Peng 1
Affiliations

Affiliations

  • 1 Institute of Orthopedics, Chinese PLA General Hospital , Beijing Key Lab of Regenerative Medicine in Orthopedics, Key Lab of Musculoskeletal Trauma & War Injuries , PLA, No. 28 Fuxing Road , Beijing 100853 , P. R. China.
  • 2 Department of Orthopedics , Tianjin Hospital , No. 406 Jiefang Nan Road , Tianjin 300211 , P. R. China.
  • 3 Department of Surgery , Ludwig-Maximilians-University , Nussbaumstr. 20 , Munich 80336 , Germany.
  • 4 State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering , Tsinghua University , Beijing 100084 , P. R. China.
  • 5 School of Biomedical Sciences , University of Hong Kong , No. 21 Sassoon Road , Pokfulam, 999077 Hong Kong , P. R. China.
PMID: 30360061 DOI: 10.1021/acsami.8b11687
Abstract

In situ tissue regeneration by homing endogenous reparative cells to the injury site has been extensively researched as a promising alternative strategy to facilitate tissue repair. In this study, a promising scaffolding system DCM-RAD/SKP, which integrated a decellularized cartilage matrix (DCM)-derived scaffold with a functionalized self-assembly Ac-(RADA)4-CONH2/Ac-(RADA)4GGSKPPGTSS-CONH2 (RAD/SKP) peptide nanofiber hydrogel, was designed for repairing rabbit osteochondral defect. In vitro experiments showed that rabbit bone marrow stem cells migrated into and have higher affinity toward the functional scaffolding system DCM-RAD/SKP than the control scaffolds. One week after in vivo implantation, the functional scaffolding system DCM-RAD/SKP facilitated the recruitment of endogenous mesenchymal stem cells within the defect site. Moreover, gene expression analysis indicated that the DCM-RAD/SKP promoted chondrogenesis of the recruited cells. In vivo results showed that the DCM-RAD/SKP achieved superior hyaline-like cartilage repair and successful subchondral bone reconstruction. By contrast, the control groups mostly led to fibrous tissue repair. These findings indicate that the DCM-RAD/SKP can recruit endogenous stem cells into the site of cartilage injury and promote differentiation of the infiltrating cells into the chondrogenic lineage, holding great potential as a one-step surgery strategy for cartilage repair.

Keywords

cartilage regeneration; decellularized extracellular matrix; endogenous stem cell; peptide.

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