2. Academic Validation
  3. Establishment of correlation between in-silico and in-vitro test analysis against Leishmania HGPRT to inhibitors

Establishment of correlation between in-silico and in-vitro test analysis against Leishmania HGPRT to inhibitors

  • Int J Biol Macromol. 2016 Feb:83:78-96. doi: 10.1016/j.ijbiomac.2015.11.051.
Md Yousuf Ansari 1,  Asif Equbal 2,  Manas Ranjan Dikhit 3,  Rani Mansuri 1,  Sindhuprava Rana 3,  Vahab Ali 2,  Ganesh Chandra Sahoo 4,  Pradeep Das 1
Affiliations

Affiliations

  • 1 Pharmacoinformatics Department, National Institute of Pharmaceutical Education and Research (NIPER), Hajipur 844102, India; BioMedical Informatics Division, Rajendra Memorial Research Institute of Medical Sciences, Agam Kuan, Patna 800007, India.
  • 2 Biochemistry Department, Rajendra Memorial Research Institute of Medical Sciences, Agam Kuan, Patna 800007, India.
  • 3 BioMedical Informatics Division, Rajendra Memorial Research Institute of Medical Sciences, Agam Kuan, Patna 800007, India.
  • 4 BioMedical Informatics Division, Rajendra Memorial Research Institute of Medical Sciences, Agam Kuan, Patna 800007, India. Electronic address: ganeshiitkgp@gmail.com.
PMID: 26616453 DOI: 10.1016/j.ijbiomac.2015.11.051
Abstract

Hypoxanthine Phosphoribosyltransferase (HGPRT; EC 2.4.2.8) is a central enzyme in the purine recycling pathway of all protozoan parasites. Protozoan parasites cannot synthesize purine Bases (DNA/RNA) which is essential for survival as lack of de-novo pathway. Thus its good target for drug design and discovery as inhibition leads to cessation of replication. PRTase (transferase enzyme) has common PRTase type I folding pattern domain for its activities. Genomic studies revealed the sequence pattern and identified highly conserved residues that catalyzed the reaction in protozoan parasites. A recombinant protein has 24 kDa molecular mass (rLdHGPRT) was cloned, expressed and purified for testing of guanosine monophosphate (GMP) analogous compounds in-vitro by spectroscopically to the rLdHGPRT, lysates protein and MTT assay on Leishmania donovani. The predicted inhibitors of different libraries were screen into FlexX. The reported inhibitors were tested in-vitro. The 2'-deoxyguanosine 5'-diphosphate (DGD) (IC50 value 12.5 μM) is two times more effective when compared to guanosine-5'-diphosphate sodium (GD). Interestingly, LdHGPRT complex has shown stable after 24 ns in molecular dynamics simulation with interacting Amino acids are Glu125, Ile127, Lys87 and Val186. QSAR studies revealed the correlation between predicted and experimental values has shown R2 0.998. Concludes that inversely proportional to their docked score with activities.

Keywords

Docking; FlexX; Leishmania; QSAR; rLdHGPRT.

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