2. Academic Validation
  3. Product profile of PEN3: the last unexamined oxidosqualene cyclase in Arabidopsis thaliana

Product profile of PEN3: the last unexamined oxidosqualene cyclase in Arabidopsis thaliana

  • Org Lett. 2009 Jun 18;11(12):2627-30. doi: 10.1021/ol9005745.
Pietro Morlacchi 1,  William K Wilson,  Quanbo Xiong,  Aparna Bhaduri,  Diana Sttivend,  Mariya D Kolesnikova,  Seiichi P T Matsuda
Affiliations

Affiliation

  • 1 Department of Chemistry, Rice University, Houston, Texas 77005, USA.
PMID: 19445469 DOI: 10.1021/ol9005745
Abstract

The triterpene product profile is reported for At5g36150 (PEN3), the last unexamined oxidosqualene cyclase in the reference plant Arabidopsis thaliana. PEN3 makes tirucalla-7,24-dien-3beta-ol ( approximately 85%) and several minor products. Also discussed are the unexpectedly facile convergent evolution of another Arabidopsis tirucalladienol synthase (LUP5), mechanistic origins of the 20S configuration, and active-site remodeling necessary to accommodate the 17alpha side chain. This work marks the first completed functional characterization of all triterpene synthases in a higher plant.

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