2. Academic Validation
  3. ADAM22 plays an important role in cell adhesion and spreading with the assistance of 14-3-3

ADAM22 plays an important role in cell adhesion and spreading with the assistance of 14-3-3

  • Biochem Biophys Res Commun. 2005 Jun 17;331(4):938-46. doi: 10.1016/j.bbrc.2005.03.229.
Pengcheng Zhu 1,  Yingying Sang,  Huanbing Xu,  Jing Zhao,  Rener Xu,  Yubo Sun,  Tianhong Xu,  Xiaolei Wang,  Linfeng Chen,  Hanping Feng,  Changben Li,  Shouyuan Zhao
Affiliations

Affiliation

  • 1 The State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai 200433, China. pzhu@cbr.med.harvard.edu
PMID: 15882968 DOI: 10.1016/j.bbrc.2005.03.229
Abstract

Cellular adhesion plays important roles in a variety of biological processes. The ADAM family contains disintegrin-like and metalloproteinase-like domains which potentially have cell adhesion and protease activities. Recent studies suggest that the interaction between 14-3-3zeta and ADAM22cyt can regulate cell adhesion and spreading, therefore it has a potential role in neural development and function. 14-3-3 family has seven highly conserved members that regulate various cellular functions. Using yeast two-hybrid method, we identified that ADAM22cyt bound some Other 14-3-3 family members. The interaction was further confirmed by in vitro protein pull-down assay and co-immunoprecipitation. We also found that the overexpression of exogenous ADAM22 in HEK293 cells could significantly enhance cell adhesion and spreading, compared with the truncated ADAM22 lack of 14-3-3 binding motifs. These results strongly demonstrated a functional role for ADAM22/14-3-3 in cell adhesion and spreading.

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