2. Dye Reagents

Dye Reagents

A dye is a colored substance that has an affinity to the substrate to which it is being applied.

A new approach is presented for analysis of microplastics in environmental samples, based on selective fluorescent staining using Nile Red (NR), followed by density-based extraction and filtration. The dye adsorbs onto plastic surfaces and renders them fluorescent when irradiated with blue light. Fluorescence emission is detected using simple photography through an orange filter. Image-analysis allows fluorescent particles to be identified and counted. Magnified images can be recorded and tiled to cover the whole filter area, allowing particles down to a few micrometres to be detected. The solvatochromic nature of Nile Red also offers the possibility of plastic categorisation based on surface polarity characteristics of identified particles. This article details the development of this staining method and its initial cross-validation by comparison with infrared (IR) microscopy. Microplastics of different sizes could be detected and counted in marine sediment samples. The fluorescence staining identified the same particles as those found by scanning a filter area with IR-microscopy.

Cyanine dyes can be used to quantify the amount of dsDNA within a sample. The linearity of fluorescence, as function of DNA amount of six dyes, is obtained by measuring the fluorescence intensity at the optimal excitation and emission maxima.

Dye Reagents (3264):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-D3010
    VF dsDNA Green Dye 98%
    VF dsDNA Green Dye is a fluorescent dye for detecting and quantifying double-stranded DNA (dsDNA). VF dsDNA Green Dye fluoresces only when bound to dsDNA, and the fluorescence intensity is proportional to the DNA concentration. VF dsDNA Green Dye does not fluoresce when bound to ssDNA, RNA, or free nucleotides. VF dsDNA Green Dye can detect dsDNA within the range of 25 pg/mL to 1000 ng/mL (Ex/Em = 480/520 nm).
    VF dsDNA Green Dye
  • HY-D3014
    Labelling Kits Centrifugation-Based Rapid Desalting Column (5KD)
    The Labeling Kits Centrifugation-Based Rapid Desalting Column (5KD) is a laboratory tool for rapid desalting, particularly suitable for the purification and concentration of proteins and other biological molecules. This product utilizes centrifugal force and passes through a specific filter membrane (with a molecular weight cut-off of 5KD) to effectively remove salts and other small molecule impurities from the sample.
    Labelling Kits Centrifugation-Based Rapid Desalting Column (5KD)
  • HY-D3015
    CY3.5 DBCO
    Cy3.5 DBCO is a bright and photostable orange-red fluorophore with a terminal DBCO group.
    CY3.5 DBCO
  • HY-P2185
    NFF-3 158584-09-9 98%
    NFF-3, the peptide, is a selective MMP substrate. NFF-3 selectively binds to MMP-3 and MMP-10 to be hydrolyzed. NFF-3 is also cleaved by trypsin, hepatocyte growth factor activator, and factor Xa. Label NFF-3 with a CyDye pair, Cy3/Cy5Q, can produce fluorescence in cell assays to detect cell activity.
    NFF-3
  • HY-P3110
    Ac-DNLD-AMC 958001-92-8 98%
    Ac-WLA-AMC is a fluorogenic substrate of caspase-3. Ac-WLA-AMC is cleaved to release the fluorescent moiety 7-amino-4-methylcoumarin (AMC), which can be used to quantify the β5c subunit activity.
    Ac-DNLD-AMC
  • HY-115402
    DAz-1 1112977-84-0 98%
    DAz-1 is a sulfonic acid probe for living cells, which can directly detect sulfonic acid-modified proteins in living cells and is cell-permeable.
    DAz-1
  • HY-118330
    Apigeninidin chloride 1151-98-0 98%
    Apigeninidin (Gesneridin) chloride, a 3‐deoxyanthocyanidin, is a fungal growth inhibitor. Apigeninidin chloride is a bioactive red biocolorant.
    Apigeninidin chloride
  • HY-123643
    Brilliant sulfaflavine 2391-30-2 98%
    Brilliant Sulfaflavine is used as a fluorescent tracer. Used to study the application rate of foliar fungicides for soybean rust control.
    Brilliant sulfaflavine
  • HY-124254
    C.I. Vat Yellow 2 129-09-9 98%
    C.I. Vat Yellow 2 is an agent of yellow dye and can also be used for the paper dyeing.
    C.I. Vat Yellow 2
  • HY-131510
    Chromoionophore XIII 135656-96-1 98%
    Chromoionophore XIII (SNARF-DE) is a pH senor that enables excitation with red light. Chromoionophore XIII functionality renders the indicator molecule lipophilic and water-insoluble but also prevents lactonization of the dye in an apolar environment.
    Chromoionophore XIII
  • HY-133376
    DBCO-NHCO-PEG12-biotin 2621391-31-7
    DBCO-NHCO-PEG12-biotin is a PEG-based PROTAC linker that can be used in the synthesis of PROTACs. DBCO-NHCO-PEG12-biotin is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.
    DBCO-NHCO-PEG12-biotin
  • HY-133532
    PB succiniMidyl ester 215868-33-0 98%
    PB succiniMidyl ester (Ocean Blue, SE) is a fluorophore with the λexcitation/emission of ~405/455 nm.
    PB succiniMidyl ester
  • HY-134564
    Fluorescein octadecyl ester 138833-46-2 98%
    Fluorescein octadecyl ester is a lipophilic fluorescent reagent is immobilized in a plasticized PVC membrane. Fluorescein octadecyl ester can reversibly recognize alcohol molecules and can be used to determine the concentration of ethanol in alcoholic drinks. Fluorescein octadecyl ester can be used as acceptor to make optrode membrane for the determination of picric acid.
    Fluorescein octadecyl ester
  • HY-135269
    Quin II potassium 73630-23-6 98%
    QUIN 2, Tetrapotassium Salt is a biochemical reagent that can be used as a biological material or organic compound for life science related research.
    Quin II potassium
  • HY-147177
    Bodipy 8-chloromethane 208462-25-3 98%
    BODIPY-8-chloromethane is a fluorophore. BODIPY-8-chloromethane can be used as as a fluorescent probe.
    Bodipy 8-chloromethane
  • HY-148958
    SNAP-Biotin 471918-16-8 98%
    SNAP-Biotin is a biotin-labeled SNAP tag probe. SNAP-Biotin can be used for protein biotinylation.
    SNAP-Biotin
  • HY-149619
    Cy5-PEG7-endo-BCN 98%
    Cy5-PEG7-endo-BCN is a dye derivative of Cyanine 5 (Cy5) (HY-D0821) containing 7 PEG units. Cy5-PEG7-endo-BCN contains the lyophilic bidentate macrocyclic ligand endo-BCN, which can further synthesize macrocyclic complexes. In click chemistry, endo-BCN can react with molecules containing azide groups to form stable triazoles in the absence of catalysts.
    Cy5-PEG7-endo-BCN
  • HY-149620
    Cy5-PEG2-exo-BCN 98%
    Cy5-PEG2-exo-BCN is a dye derivative of Cyanine 5 (Cy5) (HY-D0821) containing 2 PEG units. Cy5-PEG2-exo-BCN contains the lyophilic bidentate macrocyclic ligand BCN, which can further synthesize macrocyclic complexes. In click chemistry, BCN reacts with molecules containing azide groups to form stable triazoles in the absence of catalysts.
    Cy5-PEG2-exo-BCN
  • HY-149696
    IR-Crizotinib 2387927-74-2 98%
    IR-Crizotinib is a NF-κB-inducing kinase (NIK) inhibitor that crosses the blood-brain barrier and fluorescently localizes intracranial glioblastoma (GBM) in mice (IC50=3.381 μM). IR-Crizotinib is conjugated from the near-infrared dye IR-786 and the NF-κB-inducing kinase (NIK) inhibitor Crizotinib. IR-Crizotinib can effectively inhibit the growth and invasion of glioma in vitro and in vivo and can be used in cancer research.
    IR-Crizotinib
  • HY-151759
    Sulfo-Cy5-TCO 2129525-69-3 98%
    Sulfo-Cy5-TCO is a click chemistry reagent containing a TCO group. Sulfo-Cy5-TCO is water soluble dye, which is highly reactive with tetrazines and methyltetrazines with the fastest bioconjugation speed.
    Sulfo-Cy5-TCO