2. Apoptosis Metabolic Enzyme/Protease NF-κB Immunology/Inflammation
  3. Glutathione Peroxidase Apoptosis Ferroptosis Reactive Oxygen Species (ROS)
  4. MB-Buf

MB-Buf is a conjugation of methylene blue (HY-14536) and bufalin (HY-N0877) . MB-Buf can targeting degrade GPX4 upon light activation. MB-Buf can induce cell apoptosis, ferroptosis, and ROS production. MB-Buf can be used for the research of cancer, such as breast cancer.

For research use only. We do not sell to patients.

MB-Buf

MB-Buf Chemical Structure

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MB-Buf Related Antibodies

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Description

MB-Buf is a conjugation of methylene blue (HY-14536) and bufalin (HY-N0877) . MB-Buf can targeting degrade GPX4 upon light activation. MB-Buf can induce cell apoptosis, ferroptosis, and ROS production. MB-Buf can be used for the research of cancer, such as breast cancer[1].

IC50 & Target[1]

GPX4

 

In Vitro

MB-Buf (5 μM, 660 nm laser irradiation for 0-5 min) induces singlet oxygen, superoxide anion and hydroxyl radical generation[1].
MB-Buf (0.1-1 μM, 6 h pretreatment with 660 nm laser irradiation for 5 min) exhibits the strongest inhibitory activity against MCF-7, MDA-MB-231 and 4T1 cells with IC50 values of 28.74, 36.82 and 64.41 nM, respectively[1].
MB-Buf (0.1-1 μM, 6 h pretreatment with 660 nm laser irradiation for 5 min) induces ROS production and ferroptosis in MCF-7 cells[1].
MB-Buf (10-100 nM, 6 h pretreatment + 660 nm laser irradiation for 2-5 min) degrades GPX4 in MCF-7 cells[1].
MB-Buf (0.5 μM, 6 h pretreatment with 660 nm laser irradiation for 5 min) inhibits migration in MCF-7 cells[1].
MB-Buf (0.5 μM, 6 h pretreatment with 660 nm laser irradiation for 5 min) induces apoptosis in MCF-7 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

MB-Buf Related Antibodies

Western Blot Analysis[1]

Cell Line: MCF-7 cells
Concentration: 10, 50 and 100 nM
Incubation Time: 6 h pretreatment + 660 nm laser irradiation for 2-5 min
Result: Reduced GPX4 levels.

Immunofluorescence[1]

Cell Line: MCF-7 cells
Concentration: 0.1, 0.5 and 1 μM
Incubation Time: 6 h pretreatment with 660 nm laser irradiation for 5 min
Result: Increased ROS production.
In Vivo

MB-Buf (1 mg/kg, i.v., every 3 days) inhibits tumor growth in combination with light irradiation in 4 T1-Luci xenograft mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 4 T1-Luci xenograft mice models[1]
Dosage: 1 mg/kg
Administration: Intravenously injection, every 3 days, 660 nm laser irradiation for 5 min at 6 h post-injection
Result: Reduced tumor volume.
Had no significant changes in mouse body weight and no obvious toxicity in major organs.
Molecular Weight

774.02

Formula

C47H55N3O5S
SMILES

O=C1C=CC(C2CC[C@]3(O)C4CCC5C[C@@H](OC(CC/N=C6C7=CC=CC=C7C8=NC9=CC=C(N(CC)CC)C=C9SC8=C\6)=O)CC[C@]5(C)C4CC[C@@]32C)=CO1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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MB-Buf Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MB-BufGlutathione PeroxidaseApoptosisFerroptosisReactive Oxygen Species (ROS)Methylene blueBufalinGPX4ROSMCF-7MDA-MB-2314T1Inhibitorinhibitorinhibit

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