m7GpppAmpG ammonium  (Synonyms: m7G(5')ppp(5')(2'OMeA)pG ammonium)

m7GpppAmpG ammonium (m7G(5')ppp(5')(2'OMeA)pG ammonium) is a trinucleotide 5′ end cap analog. m7GpppAmpG ammonium binds to eIF4E with a K_D value of 45.6 nM. m7GpppAmpG ammonium caps RNA with a capping efficiency of 90%. m7GpppAmpG ammonium enhances mRNA stability and translation efficiency. m7GpppAmpG ammonium is used in mRNA therapeutic research^[1][2][3].

m7GpppAmpG ammonium enables efficient capping of RNA with 90% efficiency^[1].
m7GpppAmpG ammonium binds eIF4E with a K_D value of 45.6 nM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

m7GpppAmpG (10 μg mRNA per mouse; intramuscular injection) ammonium demonstrates lower translational efficiency and IgG antibody levels compared to novel cap analogs YK-CAP-02~04 in mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

C₃₂H₄₃N₁₅O₂₄P₄.xNH³

Liquid

Colorless to light yellow

O=P(O)(OC[C@@H]1[C@@H](OP(O)(OC[C@H]2O[C@@H](N3C(NC(N)=NC4=O)=C4N=C3)[C@H](O)[C@@H]2O)=O)[C@@H](OC)[C@H](N5C=NC6=C(N)N=CN=C65)O1)OP(OP(OC[C@H]7O[C@@H](N8C(NC(N)=N9)=C([N+](C)=C8)C9=O)[C@H](O)[C@@H]7O)([O])=O)(O)=O.[xNH3]

Room temperature in continental US; may vary elsewhere.

Solution, -20°C, 2 years

• [1]. Sikorski PJ, Warminski M, Kubacka D, et al. The identity and methylation status of the first transcribed nucleotide in eukaryotic mRNA 5' cap modulates protein expression in living cells. Nucleic Acids Res. 2020;48(4):1607-1626.  [Content Brief]

  [2]. Zhang H, et al. GPT-Driven Generation and Biological Activity Evaluation of Novel mRNA Trinucleotide Cap1 Analogs for mRNA Vaccine or Immunotherapy. Journal of Materials Chemistry B, 2025.

  [3]. Raab M, et al. Rescue of p53 functions by in vitro-transcribed mRNA impedes the growth of high-grade serous ovarian cancer. Cancer Commun (Lond). 2024 Jan;44(1):101-126.  [Content Brief]