2. Immunology/Inflammation
  3. Toll-like Receptor (TLR)
  4. Lipopolysaccharides, from S. marcescens

Lipopolysaccharides, from S. marcescens  (Synonyms: LPS, from Serratia marcescens)

Cat. No.: HY-D1056H
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Lipopolysaccharides, from S. marcescens (Serratia marcescens) are lipopolysaccharide endotoxins and TLR-4 activators derived from Serratia marcescens, classified as S-type LPS, which can activate pathogen-associated molecular patterns (PAMP) of the immune system and induce cellular secretion of migrasomes. Lipopolysaccharides, from S. marcescens exhibit a typical three-part structure: O-antigen (O-antigen), core oligosaccharide (core oligosaccharide), and lipid A (Lipid A). Lipopolysaccharides, from S. marcescens induce NF-κB activation in mouse cells via Toll-like receptor (TLR4)/MD-2. The lipopolysaccharides of S. marcescens can induce apoptosis in host immune cells, thereby suppressing the host's innate immunity.
It is recommended to prepare a stock solution of ≥2 mg/mL and ensure that it is fully mixed and dissolved. Due to the adsorption characteristics of LPS, low adsorption centrifuge tubes should be used for aliquoting and storage.

For research use only. We do not sell to patients.

Lipopolysaccharides, from S. marcescens Chemical Structure

Lipopolysaccharides, from S. marcescens Chemical Structure

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Lipopolysaccharides, from S. marcescens Related Antibodies

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Description

Lipopolysaccharides, from S. marcescens (Serratia marcescens) are lipopolysaccharide endotoxins and TLR-4 activators derived from Serratia marcescens, classified as S-type LPS, which can activate pathogen-associated molecular patterns (PAMP) of the immune system and induce cellular secretion of migrasomes. Lipopolysaccharides, from S. marcescens exhibit a typical three-part structure: O-antigen (O-antigen), core oligosaccharide (core oligosaccharide), and lipid A (Lipid A). Lipopolysaccharides, from S. marcescens induce NF-κB activation in mouse cells via Toll-like receptor (TLR4)/MD-2. The lipopolysaccharides of S. marcescens can induce apoptosis in host immune cells, thereby suppressing the host's innate immunity[1][2].
It is recommended to prepare a stock solution of ≥2 mg/mL and ensure that it is fully mixed and dissolved. Due to the adsorption characteristics of LPS, low adsorption centrifuge tubes should be used for aliquoting and storage.

IC50 & Target

TLR-4[2]
In Vitro

Note:
1. To maintain the integrity of LPS, it is recommended to store LPS solution in silanized containers. This is because LPS can adhere to plastics and certain types of glass, particularly at concentrations below 0.1 mg/mL. If concentration of LPS is higher than 1 mg/mL, this adsorption effect is relatively small, it is recommended to prepare ≥2 mg/mL stock solution and ensure sufficient mixing and dissolution. If using glass containers, ensure that the solution is thoroughly mixed for at least 30 minutes before use to redissolve any LPS that may have adsorbed to the tube walls.
2. LPS is a molecule capable of forming micelles of varying sizes in solution, and its molecular weight is not fixed. Both the stock solution and working solution can be prepared directly based on mass concentration (mg/mL, μg/mL, etc.).
3. Upon dissolution in water or PBS, LPS may form a uniformly turbid solution. For sterilization, DO NOT filter the stock solution directly. It is recommended to dilute the stock solution to the working concentration before sterilizing through a 0.22 μm filter membrane.

Lipopolysaccharides, from S. marcescens (lipid A extraction) induce NF-κB activation in mouse cells via TLR4/MD-2 but not TLR2, and stimulate the production of various inflammatory cytokines in peritoneal macrophages of C3H/HeN mice but not C3H/HeJ mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Lipopolysaccharides, from S. marcescens Related Antibodies
In Vivo

Lipopolysaccharides, from S. marcescens (lipid A extraction) have a median lethal dose of 19.2 ng/animal (i.p.) in D-GalN-sensitized C57BL/6 mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance

Solid

Color

White to off-white

SMILES

[Lipopolysaccharides, from S. marcescens]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 5 mg/mL (Need ultrasonic and warming; DMSO can inactivate Lipopolysaccharides, from S. marcescens's activity)

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The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
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Lipopolysaccharides, from S. marcescens Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Lipopolysaccharides, from S. marcescensLPS, from Serratia marcescensToll-like Receptor (TLR)Inhibitorinhibitorinhibit

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