2. Immunology/Inflammation
  3. Toll-like Receptor (TLR)
  4. Lipopolysaccharides, from E. coli O26:B6

Lipopolysaccharides, from E. coli O26:B6  (Synonyms: LPS, from Escherichia coli (O26:B6))

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Lipopolysaccharides, from E. coli (Escherichia coli) O26:B6 are lipopolysaccharide endotoxins and TLR-4 activators derived from E. coli, classified as S-type LPS, which can activate pathogen-associated molecular patterns (PAMP) of the immune system and induce cellular secretion of migrasomes. Lipopolysaccharides, from E. coli O26:B6 exhibit a typical three-part structure: O-antigen (O-antigen), core oligosaccharide (core oligosaccharide), and lipid A (Lipid A), and can be recognized by the core-specific monoclonal antibody MAb J8-4C10. Lipopolysaccharides, from E. coli O26:B6 can promote an increase in pro-inflammatory cytokines in plasma, thereby triggering hypothalamic-pituitary-adrenal (HPA) activation and leading to adrenal oxidative damage. The pathogenic effects of Lipopolysaccharides, from E. coli O26:B6 can be blocked by PD149163 (HY-123434).
It is recommended to prepare a stock solution of ≥2 mg/mL and ensure that it is fully mixed and dissolved. Due to the adsorption characteristics of LPS, low adsorption centrifuge tubes should be used for aliquoting and storage.

For research use only. We do not sell to patients.

Lipopolysaccharides, from E. coli O26:B6 Chemical Structure

Lipopolysaccharides, from E. coli O26:B6 Chemical Structure

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Lipopolysaccharides, from E. coli O26:B6 Related Antibodies

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Description

Lipopolysaccharides, from E. coli (Escherichia coli) O26:B6 are lipopolysaccharide endotoxins and TLR-4 activators derived from E. coli, classified as S-type LPS, which can activate pathogen-associated molecular patterns (PAMP) of the immune system and induce cellular secretion of migrasomes. Lipopolysaccharides, from E. coli O26:B6 exhibit a typical three-part structure: O-antigen (O-antigen), core oligosaccharide (core oligosaccharide), and lipid A (Lipid A), and can be recognized by the core-specific monoclonal antibody MAb J8-4C10. Lipopolysaccharides, from E. coli O26:B6 can promote an increase in pro-inflammatory cytokines in plasma, thereby triggering hypothalamic-pituitary-adrenal (HPA) activation and leading to adrenal oxidative damage. The pathogenic effects of Lipopolysaccharides, from E. coli O26:B6 can be blocked by PD149163 (HY-123434)[1][2][3].
It is recommended to prepare a stock solution of ≥2 mg/mL and ensure that it is fully mixed and dissolved. Due to the adsorption characteristics of LPS, low adsorption centrifuge tubes should be used for aliquoting and storage.

IC50 & Target

TLR-4[2]
In Vitro

Note:
1. To maintain the integrity of LPS, it is recommended to store LPS solution in silanized containers. This is because LPS can adhere to plastics and certain types of glass, particularly at concentrations below 0.1 mg/mL. If concentration of LPS is higher than 1 mg/mL, this adsorption effect is relatively small, it is recommended to prepare ≥2 mg/mL stock solution and ensure sufficient mixing and dissolution. If using glass containers, ensure that the solution is thoroughly mixed for at least 30 minutes before use to redissolve any LPS that may have adsorbed to the tube walls.
2. LPS is a molecule capable of forming micelles of varying sizes in solution, and its molecular weight is not fixed. Both the stock solution and working solution can be prepared directly based on mass concentration (mg/mL, μg/mL, etc.).
3. Upon dissolution in water or PBS, LPS may form a uniformly turbid solution. For sterilization, DO NOT filter the stock solution directly. It is recommended to dilute the stock solution to the working concentration before sterilizing through a 0.22 μm filter membrane.

Lipopolysaccharides, from E. coli O26:B6 are smooth-type LPS, which can be purified using the phenol/water method, while other rough-type LPS can be purified using the phenol/chloroform/petroleum ether method[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Lipopolysaccharides, from E. coli O26:B6 Related Antibodies
Appearance

Solid

Color

White to off-white

SMILES

[Lipopolysaccharides, from E. coli O26:B6]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (Need ultrasonic; DMSO can inactivate Lipopolysaccharides, from E. coli O26:B6's activity)

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Lipopolysaccharides, from E. coli O26:B6 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Lipopolysaccharides, from E. coli O26:B6LPS, from Escherichia coli (O26:B6)Toll-like Receptor (TLR)Inhibitorinhibitorinhibit

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