Lipopolysaccharides, from S. enterica serotype minnesota Re 595 (Re mutant)  (Synonyms: LPS, from Salmonella enterica (Serotype minnesota Re 595 (Re mutant)))

Lipopolysaccharides (LPS), from S. enterica (Salmonella enterica) serotype minnesota Re 595 (Re mutant) is prepared from Salmonella enterica strain Re 595 (Re mutant). The structure in the LPS of strain Re 595 was shown to induce secretion and aggregation in human platelets^[1].
It is recommended to prepare a stock solution of ≥2 mg/mL and ensure that it is fully mixed and dissolved. Due to the adsorption characteristics of LPS, low adsorption centrifuge tubes should be used for aliquoting and storage.

Note:
1. To maintain the integrity of LPS, it is recommended to store LPS solution in silanized containers. This is because LPS can adhere to plastics and certain types of glass, particularly at concentrations below 0.1 mg/mL. If concentration of LPS is higher than 1 mg/mL, this adsorption effect is relatively small, it is recommended to prepare ≥2 mg/mL stock solution, vortex thoroughly for more than 10 minutes, and use ultrasound to assist dissolution if necessary, and ensure sufficient mixing and dissolution. If using glass containers, ensure that the solution is thoroughly mixed for at least 30 minutes before use to redissolve any LPS that may have adsorbed to the tube walls.
2. LPS is a molecule capable of forming micelles of varying sizes in solution, and its molecular weight is not fixed. Both the stock solution and working solution can be prepared directly based on mass concentration (mg/mL, μg/mL, etc.).
3. Upon dissolution in water or PBS, LPS may form a uniformly turbid solution. For sterilization, DO NOT filter the stock solution directly. It is recommended to dilute the stock solution to the working concentration before sterilizing through a 0.22 μm filter membrane.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Solid

White to light yellow

[Lipopolysaccharides, from S. enterica serotype minnesota Re 595 (Re mutant)]

Room temperature in continental US; may vary elsewhere.

• [1]. Timmons S, et al. Mechanism of human platelet activation by endotoxic glycolipid-bearing mutant Re595 of Salmonella minnesota. Blood. 1986 Nov;68(5):1015-23.  [Content Brief]