2. Induced Disease Models Products Immunology/Inflammation Metabolic Enzyme/Protease
  3. Immunology and Inflammatory Disease Models NO Synthase Endogenous Metabolite
  4. Pancreatitis Models
  5. L-Arginine

L-Arginine ((S)-(+)-Arginine) is the substrate for the endothelial nitric oxide synthase (eNOS) to generate NO. L-Arginine is transported into vascular smooth muscle cells by the cationic amino acid transporter family of proteins where it is metabolized to nitric oxide (NO), polyamines, or L-proline. L-Arginine is a potent vasodilator, and can be used to induce experimental acute pancreatitis.

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L-Arginine Chemical Structure

L-Arginine Chemical Structure

CAS No. : 74-79-3

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10 mM * 1 mL in Water
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Based on 12 publication(s) in Google Scholar

Other Forms of L-Arginine:

L-Arginine Related Antibodies

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nNOS iNOS eNOS

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Description

L-Arginine ((S)-(+)-Arginine) is the substrate for the endothelial nitric oxide synthase (eNOS) to generate NO. L-Arginine is transported into vascular smooth muscle cells by the cationic amino acid transporter family of proteins where it is metabolized to nitric oxide (NO), polyamines, or L-proline. L-Arginine is a potent vasodilator, and can be used to induce experimental acute pancreatitis[1][2][3][4][5].

IC50 & Target

Microbial Metabolite

 

Human Endogenous Metabolite

 

eNOS

 

Cellular Effect
Cell Line Type Value Description References
HEK293 EC50
283 μM
Compound: 6322
Substrate uptake by the Cationic Amino Acid Transporter (CAT-3, SLC7A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC7A3 cells
Substrate uptake by the Cationic Amino Acid Transporter (CAT-3, SLC7A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC7A3 cells
10.5281/zenodo.6782708
In Vitro

Arginine is an α-amino acid. The L-form is one of the 20 most common natural amino acids. At the level of molecular genetics, in the structure of the messenger ribonucleic acid mRNA, CGU, CGC, CGA, CGG, AGA, and AGG, are the triplets of nucleotide bases or codons that code for arginine during protein synthesis. In mammals, arginine is classified as a semiessential or conditionally essential amino acid, depending on the developmental stage and health status of the individual[1].
L-Arginine is associated with a decrease in cardiac index. L-Arginine (450 mg/kg during a 15-minute period) amplifies and sustains the hyperemia (38%) and increases absolute brain blood flow after eNOS upregulation by chronic Simvastatin (HY-17502) (2 mg/kg; sc; daily for 14 days) in SV-129 mice[2][3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

L-Arginine Related Antibodies
In Vivo

L-Arginine has been widely accepted as a method to induce experimental acute pancreatitis[4][5].
Dose reference for L-Arginine induction[4][5]:
(1) Model animal: Male Wistar albino rats
Acute pancreatitis: single i.p. injection of 500 mg of L-arginine/100 g body weight
(2) Model animal: Male ICR mice
Acute pancreatitis: i.p. with 2.25 g/kg body weight of L-Arginine hourly for 2 hours
Dissolution method of L-Arginine[5]:
L-arginine solution was prepared by dissolving L-arginine powder in 0.9% normal saline and adjusting the pH to 7 with 5 N HCl.
Induction of Experimental Acute Pancreatitis[4][5]
Background
L-Arginine is a substrate for endothelial nitric oxide synthase (eNOS) to produce NO, which can be metabolized into nitric oxide (NO), polyamines or L-proline, stimulating inflammatory responses. L-Arginine can also selectively destroy pancreatic acinar cells, leading to acute necrotizing pancreatitis.
Specific Modeling Methods
Rat[4]: Wistar albino rats • Male • 150 g
Administration: 250 mg, 500 mg, 750 mg every 100 g body weight • ip • single dose • starved for 15 hours
Mice[5]: ICT mouse • Male • 4-week-old • 25-30 g
Administration: 450 mg/100 g body weight • ip • twice dosages • sacrificed at 72 hours
Note
(1) When the dose exceeds 500 mg/100 g body weight, most rats will die within a few hours after injection; When the dose is less than 250 mg, the induced symptoms of pancreatic disease will be mild[4].
(2) Dissolution method in Reference: L-Arginine solution was prepared by dissolving L-Arginine powder in 0.9% normal saline and adjusting pH to 7 with 5N hydrochloric acid[5].
Modeling Indicators
Phenotypic changes: weight loss, lower activity, softer hair.
Tissue morphology: pancreatic edema, damage, atrophy, granulation tissue contraction and chalky spots (location of fat necrosis).
Cellular changes: basophils decrease and vesicles, cell pyknosis, nuclear fragmentation. Pancreatic albino cells disappear and die, and pancreatic tissue is replaced by monocytes and fibroblasts.
Molecular changes: serum amylase levels increase, and fat necrosis is obvious.
Correlated Product(s): Curcumin (HY-N0005)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

174.20

Formula

C6H14N4O2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

N[C@@H](CCCNC(N)=N)C(O)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Store at room temperature 3 years

In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (287.03 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.7405 mL 28.7026 mL 57.4053 mL
5 mM 1.1481 mL 5.7405 mL 11.4811 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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Concentration (final)

C2

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Volume (final)

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In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (574.05 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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g

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(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.89%

SDS (393 KB)

COA (242 KB) HNMR (234 KB) RP-HPLC (242 KB) MS (68 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 5.7405 mL 28.7026 mL 57.4053 mL 143.5132 mL
5 mM 1.1481 mL 5.7405 mL 11.4811 mL 28.7026 mL
10 mM 0.5741 mL 2.8703 mL 5.7405 mL 14.3513 mL
15 mM 0.3827 mL 1.9135 mL 3.8270 mL 9.5675 mL
20 mM 0.2870 mL 1.4351 mL 2.8703 mL 7.1757 mL
25 mM 0.2296 mL 1.1481 mL 2.2962 mL 5.7405 mL
30 mM 0.1914 mL 0.9568 mL 1.9135 mL 4.7838 mL
40 mM 0.1435 mL 0.7176 mL 1.4351 mL 3.5878 mL
50 mM 0.1148 mL 0.5741 mL 1.1481 mL 2.8703 mL
60 mM 0.0957 mL 0.4784 mL 0.9568 mL 2.3919 mL
80 mM 0.0718 mL 0.3588 mL 0.7176 mL 1.7939 mL
100 mM 0.0574 mL 0.2870 mL 0.5741 mL 1.4351 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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L-Arginine Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L-Arginine74-79-3NO SynthaseEndogenous MetaboliteNitric oxide synthasesNOSNOvascularsmoothmuscletransporterInhibitorinhibitorinhibit

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