2. MCE Kits
  3. Molecular Biology
  4. PCR & qPCR
  5. Real-Time PCR
  6. SYBR Green qPCR Master Mix

SYBR Green qPCR Master Mix 

Cat. No.: HY-K0501
Manual COA SDS Technical Support

MCE qPCR Master Mix is provided as a simple-to-use, stabilized 2× formulation that includes all components for qPCR except sample DNA, primers and water.

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237 Publications Citing Use of MCE SYBR Green qPCR Master Mix

  • Description

  • Storage

  • Components

  • Documentation

Description
& Advantages

The Super Hot-Start SYBR Green qPCR Master Mix is a ready-to-use qPCR cocktail. It contains a novel Taq DNA Polymerase, unique hot start reagents, optimized buffer, SYBR Green I, dNTPs, PCR Enhancer and PCR stabilizer. qPCR Master Mix is provided at 2× concentration and can be used at 1× concentration by adding template, primer, passive reference dye (optional) and ddH2O.

MCE qPCR Master Mix can be directly used for robust and low-template quantitative PCR with high sensitivity, specificity and reliability. Two separate tubes of ROX Reference Dye are included for use with instruments that require a high or low level of reference dye for rectify the error of fluorescence signals between different wells.

 

1. Ready to use.

2. Optimized buffer enhances specificity and reduces primer-dimer formation.

3. High sensitivity, specificity and reliability.

4. Passive reference dyes are provided for different qPCR instruments.
Storage

-20°C, 2 years

Protecting from light, and avoid repetitive freeze-thaw cycles
Components
Components HY-K0501-100rxns HY-K0501-500rxns
SYBR Green qPCR Master Mix (2×) 1 mL 1 mL × 5
ROX Reference Dye (50×) 40 μL 40 μL × 5
ROX Reference Dye Ⅱ (50×) 40 μL 40 μL × 5
Documentation

Manual (364 KB) SDS (201 KB)
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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