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  2. Protein Biology
  3. Protein Purification
  4. Agarose Beads
  5. Phenyl Agarose (High Sub) 6FF

Phenyl Agarose (High Sub) 6FF  

Cat. No.: HY-K0269
Manual COA SDS Technical Support

MCE Phenyl Agarose (High Sub) 6FF is a high-substitution hydrophobic chromatography medium formed by covalently coupling phenyl ligands to agarose. It is suitable for laboratory-scale and industrial-scale purification of biomolecules with relatively weak hydrophobicity.

Phenyl Agarose (High Sub) 6FF
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  • Description

  • Storage

  • Attention

  • Documentation

Description
& Advantages

Hydrophobic Interaction Chromatography (HIC) media are designed to purify biomolecules based on differences in surface hydrophobicity. Common ligands such as butyl or phenyl are covalently attached to agarose or synthetic resins. Proteins bind in high-salt conditions and are gently eluted by decreasing salt concentration, preserving structure and activity. HIC is widely used in downstream processing of peptides, antibodies, and protein therapeutics, from lab-scale to industrial applications.

MCE Phenyl Agarose (High Sub) 6FF is a high-substitution hydrophobic chromatography medium formed by covalently coupling phenyl ligands to agarose. It features high mechanical strength, fast flow rate, strong selectivity, high recovery of biological activity, and environmental safety. The medium is resistant to strong acids and bases, allowing for in-place cleaning (CIP) and high-temperature sterilization. Compared to reverse-phase chromatography media, it has a lower ligand density and milder elution conditions, which better preserve the biological activity of target molecules. It is suitable for laboratory-scale and industrial-scale purification of biomolecules with relatively weak hydrophobicity.

Each 1 mL of total volume contains 0.5 mL of resin. Thoroughly resuspend the resin before use and mix well before aliquoting.

 

The specifications of the product correspond to the actual resin volume, with the resin content of 50%.

Storage

4°C, 5 years

Do not dry or freeze

Attention

1. Ensure the resin is thoroughly resuspended before use.

2. It is recommended to replace the storage buffer every 2-3 months to prevent microbial contamination due to ethanol evaporation.

3. This product is for R&D use only, not for drug, household, or other uses.

4. For your safety and health, please wear a lab coat and disposable gloves to operate.

Documentation

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Phenyl Agarose (High Sub) 6FF
Cat. No.:
HY-K0269
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