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  5. In vivo OptiLNP RNA Transfection Reagent (Lung-targeted)

In vivo OptiLNP RNA Transfection Reagent (Lung-targeted) 

Cat. No.: HY-K2025
Manual COA Technical Support

MCE In vivo OptiLNP RNA Transfection Reagent (Lung-targeted) is a ready-to-use transfection reagent based on LNP technology. It is designed for the efficient transfection of mRNA and short-chain RNA via intravenous injection in experimental animals.

Size Price Stock Quantity
0.3 mL In-stock
0.5 mL In-stock
1 mL In-stock

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In vivo OptiLNP RNA Transfection Reagent (Lung-targeted) Featured Recommendations:

    MedChemExpress Validation
    Balb/c mice were injected with Fluc mRNA-LNP complexes via the tail vein at a single dose of 0.5 mpk (10 μg per mouse). Animal imaging was conducted 3 hours after administration to observe tissue distribution. The In vivo OptiLNP RNA Transfection Reagent (Lung-targeted) (HY-K2025) achieved lung-targeted delivery with a targeting efficiency higher than 95% and hardly any off-target escape.

    • Description

    • Storage

    • Protocol

    • Attention

    • Components

    • Documentation

    Description
    & Advantages

    Lipid nanoparticles (LNP) are a lipid-based nanoscale drug delivery system widely used for the delivery of nucleic acids (such as mRNA, siRNA, DNA, etc.) and small molecule drugs.

    Naked RNA is a negatively charged hydrophilic macromolecule that faces challenges in entering cells due to electrostatic repulsion from the cell membrane and is easily degraded by RNases. By encapsulating RNA within LNP, RNA can effectively traverse the cell membrane and be released into the cytoplasm, enabling efficient delivery.

    MCE In vivo OptiLNP RNA Transfection Reagent (Lung-targeted) is a ready-to-use transfection reagent based on LNP technology. It utilizes the unique transmembrane transport and escape mechanism of LNP to achieve efficient delivery of RNA, facilitating Lung-targeted delivery of mRNA and short-chain RNA via intravenous injection in experimental animals.

     

    Features of MCE In vivo OptiLNP RNA Transfection Reagent (Lung-targeted)

    1. Lipid Nanoparticle (LNP) Technology: Utilizes LNP carriers to effectively protect RNA from degradation and immune system clearance during the delivery process.

    2. Efficient Intracellular Delivery: The unique transmembrane transport and escape mechanisms of LNPs achieve an encapsulation rate of over 80% for small sample amount.

    3. High Delivery Efficiency: RNA Lung delivery efficiency > 85%.

    4. Wide Applicability: Supports a variety of RNA types such as siRNA, miRNA, gRNA, mRNA.

    5. User-Friendly Operation: No encapsulation equipment is required; RNA-LNP encapsulation and transfection can be completed in a single mixing step.

    6. Biocompatibility: Extremely low toxicity in animals with mild effects.

    7. Safety: The formulation is ethanol-free and uses biodegradable lipid materials to ensure safety.

    Storage

    Transfection Reagent (Lung-targeted), Dilution Buffer (Lung-targeted): 4℃, 1 year. Do not freeze.

    Fluc mRNA Positive Control (Lyophilized, Lung-targeted): -20℃, 1 year.

    Protocol
    Protocol

    1. Prepare RNA Pre-mix Solution: Dilute the mRNA stock solution with the dilution buffer.

    2. Prepare RNA-LNP Complex: Add the transfection reagent to the RNA pre-mixed solution and mix thoroughly by pipetting up and down at least 10 times.

    3. Injection: Inject the RNA-LNP complex into the animal.

     

    Recommended Dosage

    1. The intravenous injection dosage for mice is generally 10 mL/kg.

    2. It is recommended that the stock concentration of mRNA be ≥ 1 μg/μL, and the concentration of mRNA in the RNA-LNP complex be ≤ 0.2 μg/μL. For siRNA, the stock concentration should be ≥ 1 μg/μL, and the concentration of siRNA in the RNA-LNP complex should be ≤ 0.3 μg/μL.

     

    Analysis of Transfection Efficiency

    The delivery and transfection efficiency can be evaluated using conventional detection methods, including:

    Fluorescence imaging system: Detecting fluorescence signals to assess delivery efficiency.

    RT-qPCR: Quantifying target gene expression levels.

    Western Blot: Detecting target protein expression.

    Immunofluorescence: Localizing target protein expression.

    Additionally, tissue sampling and distribution analysis, as well as animal phenotype and physiological analysis, should be performed to evaluate the transfection delivery effectiveness.

    Attention

    1. This product has been filtered using a 0.22 μm membrane.

    2. The Fluc mRNA Positive Control is provided as a lyophilized powder. Please centrifuge before opening the cap. Use nuclease-free water to dissolve and prepare a positive control stock solution at 1 μg/μL. The stock solution can be stored at -80°C for 6 months, avoiding repeated freeze-thaw cycles.

    3. For optimal transfection efficiency, it is recommended to dissolve the RNA stock in nuclease-free sterile water. Other solvents may affect transfection efficiency. It is recommended to use commercially available RNA; for self-prepared RNA, purification and desalting are necessary to prevent RNA precipitation.

    4. It is recommended to prepare RNA pre-mixes using the dilution buffer provided with the kit to dilute the RNA stock solution.

    5. Common short-chain RNAs (e.g., siRNA) typically weigh between 13 - 15 μg per 1 nmol without special modifications. The exact weight can be calculated based on the specific nucleic acid sequence and modifications.

    6. RNA-LNP complexes can be stably stored at room temperature for up to 8 h. Do not store RNA-LNP complexes under freezing conditions.

    7. If dilution of the RNA-LNP complex is required, it is recommended to use the dilution buffer provided with the kit for dilution.

    8. Under suitable conditions, repeated transfection administration can also be performed.

    9. To avoid RNAse contamination, RNA-free enzymes and non-pyrogenic materials should be used throughout the experiment.

    10. This product is for R&D use only, not for drug, household, or other uses.

    11. For your safety and health, please wear a lab coat and disposable gloves to operate.

    Components
    Cat. No. Components HY-K2025
    -0.3 mL
    HY-K2025
    -0.5 mL
    HY-K2025
    -1 mL
    Storage
    HY-K2025-A Transfection Reagent
    (Lung-targeted)
    0.3 mL 0.5 mL 1 mL 4℃, 1 year
    Do not freeze
    HY-K2025-B Fluc mRNA Positive Control
    (Lyophilized, Lung-targeted)
    10 μg 10 μg 10 μg -20℃, 1 year
    HY-K2025-C Dilution Buffer
    (Lung-targeted)
    8 mL 8 mL × 2 8 mL × 3 4℃, 1 year
    Do not freeze
    Documentation

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    In vivo OptiLNP RNA Transfection Reagent (Lung-targeted)
    Cat. No.:
    HY-K2025
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