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  6. siRNA/miRNA Transfection Reagent

siRNA/miRNA Transfection Reagent 

Cat. No.: HY-K2017
Manual COA SDS Technical Support

MCE siRNA/miRNA Transfection Reagent is a novel cationic polymer transfection reagent for siRNA and miRNA transfection.

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    MedChemExpress Validation
    Experimental conditions:
    Cell density: 40% density during cell transfection
    Dosage: 1 μL siRNA (EGFP/NC, 20 μM) + 2 μL transfection reagent (HY-K2017)
    Detection scheme: 36 h after siRNA transfection, use fluorescence microscopy to detect the interference effect of EGFP green fluorescent protein
    siRNA/miRNA Transfection Reagent (HY-K2017) demonstrated ​​high siRNA transfection efficiency and significant EGFP silencing in both A549 and HT29 cells.​

    MedChemExpress Validation
    Experimental Conditions:
    Cell Density: 40% of the cell density at the time of transfection
    Dosage: 2 μL siRNA (MALT1/NC, 20 μM) + 4 μL transfection reagent
    Assay: 36 hours after siRNA transfection, RNA was extracted using Trizol and reverse transcribed, followed by qPCR verification of gene silencing efficacy.
    The results demonstrate that the ​​siRNA/miRNA Transfection Reagent (HY-K2017)​​ achieves ​​significant silencing efficacy​​ against the ​​MALT1 gene​​ in ​​Caco-2 cells​​.

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    • Storage

    • Components

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    Description
    & Advantages

    RNA interference (RNAi) refers to the small double-stranded RNAs that can specifically degrade homologous mRNAs, inhibiting or shutting down the expression of the corresponding genes. By utilizing RNAi technology to design small interfering RNA (siRNA) targeting the mRNA of disease-causing genes, it is possible to specifically knock out or turn off the expression of specific genes.

     

    MCE siRNA/miRNA Transfection Reagent is a novel cationic polymer transfection reagent for siRNA and miRNA transfection. The exogenous siRNA/miRNA forms a stable complex with the transfection reagent and enters the cell through cytosis, subsequently releasing the siRNA/miRNA in the cytoplasm to achieve cellular transfection of siRNA/miRNA.

     

    Features of MCE siRNA/miRNA Transfection Reagent

    1. Wide range of applications: High transfection efficiency in many common cell types, suitable for siRNA/miRNA-mediated gene suppression experiments.

    2. High transfection efficiency: Efficient transfection of primary cells with high cell viability.

    3. Low cytotoxicity: Mild action and biocompatible.

    4. Simple operation: No need to remove transfection complexes or change medium after transfection.
    Storage

    -20℃, 1 year

    Avoid repeat freeze-thaw cycles
    Components
    Components HY-K2017-1 mL HY-K2017-5 mL
    siRNA/miRNA Transfection Reagent 1 mL 1 mL × 5
    Documentation

    Manual (1222 KB) SDS (340 KB)
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    siRNA/miRNA Transfection Reagent
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