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  5. MBP Agarose (Dextrin) 6FF

MBP Agarose (Dextrin) 6FF 

Cat. No.: HY-K0252
Manual COA SDS Technical Support

MCE MBP Agarose (Dextrin) 6FF is prepared by covalently coupling dextrin to an agarose matrix. It features high binding capacity, excellent specificity, and superior ligand stability. It can achieve one-step purification of MBP fusion protein.

MBP Agarose (Dextrin) 6FF
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  • Description

  • Storage

  • Attention

  • Documentation

Description
& Advantages

Maltose Binding Protein (MBP) is a maltose/maltodextrin-binding protein derived from Escherichia coli, with a molecular weight of approximately 42 kDa.

The MBP tag offers the following advantages: it significantly enhances the solubility of heterologous proteins, particularly those expressed as inclusion bodies; it can be expressed in Escherichia coli or yeast systems to improve the expression level of target proteins; it promotes proper protein folding; the MBP tag can be removed by specific proteases such as PreScission, TEV, or Thrombin; it enables a simple and highly specific purification process. Additionally, the MBP tag is suitable for studies involving protein expression, localization, purification, and immunoassays. Due to these advantages, the MBP tag is widely used in protein research.

MCE MBP Agarose (Dextrin) 6FF is prepared by covalently coupling dextrin to an agarose matrix. It features high binding capacity, excellent specificity, and superior ligand stability. This resin enables one-step purification of MBP fusion proteins. The bound fusion protein can be gently eluted using 10 mM maltose, which helps preserve the biological activity of the target protein. The MBP tag can be subsequently removed by site-specific protease cleavage.

Each 1 mL of total volume contains 0.5 mL of resin. Thoroughly resuspend the resin before use and mix well before aliquoting.

 

The specifications of the product correspond to the actual resin volume, with the resin content of 50%.

Storage

4°C, 2 years

Do not dry or freeze

Attention

1. Ensure the resin is thoroughly resuspended before use.

2. It is recommended to replace the storage buffer every 2-3 months to prevent microbial contamination due to ethanol evaporation.

3. To minimize protein degradation, protease inhibitor cocktails (MCE Cat. No. HY-K0010, HY-K0011) are highly recommended.

4. This product is for R&D use only, not for drug, household, or other uses.

5. For your safety and health, please wear a lab coat and disposable gloves to operate.

Documentation

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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MBP Agarose (Dextrin) 6FF
Cat. No.:
HY-K0252
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