2. Apoptosis
  3. Caspase Apoptosis
  4. IGN523

IGN523 is an anti-CD98 antibody (hCD98, KD = 0.55 nM). IGN523 induces antibody-dependent cell-mediated cytotoxicity (ADCC) activity, lysosomal membrane permeabilization, and inhibition of essential amino acid transport, ultimately leading to caspase-3 and caspase-7-mediated apoptosis of tumor cells. IGN523 inhibits tumor growth in multiple tumor xenograft models. IGN523 is useful in the research of non-small cell lung cancer (NSCLC), acute myeloid leukemia (AML), and other cancers..

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IGN523

IGN523 Chemical Structure

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Description

IGN523 is an anti-CD98 antibody (hCD98, KD = 0.55 nM). IGN523 induces antibody-dependent cell-mediated cytotoxicity (ADCC) activity, lysosomal membrane permeabilization, and inhibition of essential amino acid transport, ultimately leading to caspase-3 and caspase-7-mediated apoptosis of tumor cells. IGN523 inhibits tumor growth in multiple tumor xenograft models. IGN523 is useful in the research of non-small cell lung cancer (NSCLC), acute myeloid leukemia (AML), and other cancers.[1].

IC50 & Target[1]

Caspase 3

 

Caspase-7

 

In Vitro

IGN523 elicits strong ADCC activity in Ramos, KG-1 and OCI-AML-3 cells, with EC50s of 18.6 ng/mL, 73.8 ng/mL and 83.3 ng/mL, respectively, does not reduce ADCC activity in IGN523 cells[1].
IGN523 (2 µg/mL, 30 min/48 h) elicits Caspase-dependent apoptosis, increases lysosomal membrane permeability and decreases amino acid transport in Ramos, KG-1 and OCI-AML-3 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

IGN523 Related Antibodies

Apoptosis Analysis[1]

Cell Line: Ramos, KG-1 and OCI-AML-3 cells
Concentration: 2 µg/mL
Incubation Time: 48 h
Result: Decreased cell viability in the presence of AbXL (10 µg/mL) (Ramos: 70% reduction; OCI-AML-3: 76% reduction; KG-1: 80% reduction) , but not when used alone.
Increased caspase-3 and caspase-7 activity (Ramos: 3.3-fold; OCI-AML-3: 7.5-fold and KG-1: 6.6-fold).

Immunofluorescence[1]

Cell Line: Ramos cells
Concentration: 2 µg/mL
Incubation Time: 4℃ for 30 min
Result: Showed the CD98-IGN523 complex localized to lysosome-associated membrane protein LAMP-1 structures, increased the rate of internalization, capped CD98 at the cell surface.
In Vivo

IGN523 (15 mg/kg, i.p., once a week, twice/three times) inhibits tumor growth in Ramos and Dau xenografts mice model[1].
IGN523 (15 mg/kg, i.p., once a week, twice/three times/four times) inhibits tumor growth in AML and primary NSCLC xenograft mice models[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Ramos and Dau xenografts mice model[1]
Dosage: 15 mg/kg
Administration: i.p., once a week, twice/three times
Result: Inhibited tumor growth, with TGIs of 98%, 76 %, respectively.
Animal Model: AML (HL-60, OCI-AML-3 (NOD/SCID) and KG-1 (1 x 107) and NSCLC (IGN-LNG-12 and IGN-LNG-54) (1 x 106) xenografts NOD/SCID or CB.17-SCID mice model[1]
Dosage: 15 mg/kg
Administration: i.p., once a week, twice/three times/four times
Result: Reduced tumor growth compared with isotype control in all 5 xenograft models, with TGIs of HL-60 (94%), OCI-AML-3 (74%), KG-1 (53%), IGN-LNG-12 (44%) and IGN-LNG-54 (56%), without inducing body weight loss. Completely eliminated CD98 expression and CD98+-tumor cells.
Gene ID

6520  [NCBI]

SMILES

N/A
Shipping

Room temperature in continental US; may vary elsewhere.
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Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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IGN523 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

IGN523IGN 523IGN-523CaspaseApoptosisAntibodyapoptosisNSCLCAMLRamos cellsKG-1 cellsOCI-AML-3 cellsNOD/SCID or CB.17-SCID miceInhibitorinhibitorinhibit

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