2. Neuronal Signaling NF-κB Metabolic Enzyme/Protease Immunology/Inflammation Apoptosis Autophagy Anti-infection
  3. α-synuclein Reactive Oxygen Species (ROS) Apoptosis Autophagy Parasite
  4. Hupehenine

Hupehenine is an orally active isosteroidal alkaloid that can be extracted from F. hupehensis. Hupehenine exhibits activities such as antitussive, expectorant, anticancer, and antiparasitic. In vitro, Hupehenine can also inhibit α-synuclein seeded fibril formation, making it applicable for the research of Parkinson's disease and other related disorders.

For research use only. We do not sell to patients.

Hupehenine Chemical Structure

Hupehenine Chemical Structure

CAS No. : 98243-57-3

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Hupehenine:

Hupehenine Related Antibodies

Top Publications Citing Use of Products

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Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Hupehenine is an orally active isosteroidal alkaloid that can be extracted from F. hupehensis. Hupehenine exhibits activities such as antitussive, expectorant, anticancer, and antiparasitic. In vitro, Hupehenine can also inhibit α-synuclein seeded fibril formation, making it applicable for the research of Parkinson's disease and other related disorders[1][2][3][4].

In Vitro

Hupehenine (24 h) can inhibit α-synuclein seeded fibril formation in vitro[1].
Hupehenine (2-10 µM; 48 h) does not significantly reduce α-synuclein seed-induced cytotoxicity in SH-SY5Y cells[1].
Hupehenine (7.2-14.4 µM; 48 h) can induce the elevation of ROS levels, loss of mitochondrial membrane potential, cell cycle arrest, as well as autophagy and apoptosis in Leishmania donovani promastigotes[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Hupehenine Related Antibodies

Real Time qPCR[2]

Cell Line: Leishmania donovani
Concentration: 7.2 µM
Incubation Time: 48 h
Result: Increased the levels of major autophagic genes ATG7 and ATG8B.
In Vivo

Pharmacokinetic Analysis in Rats[3]

Route Dose (mg/kg) AUC0-t (ng/mL h) AUC0-∞ (ng/mL h) t1/2 (h) CL (L/h/kg) V (L/kg) Cmax (ng/mL) Bioavailability, F
p.o. 10 935.6 1097.4 5.4 11.5 91.5 390.2 13.4%
i.v. 5 3494.9 3514.3 4..6 1.5 9.9 1400.5

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

415.65

Formula

C27H45NO2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1CC[C@@]2(C)[C@]([C@H](O)C[C@]3([H])[C@]2([H])C[C@]4([H])[C@@]3([H])CC[C@]5([H])[C@@]4([H])CN6[C@](CC[C@H](C)C6)([H])[C@@H]5C)([H])C1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (240.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4059 mL 12.0294 mL 24.0587 mL
5 mM 0.4812 mL 2.4059 mL 4.8117 mL
View the Complete Stock Solution Preparation Table

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (6.01 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (6.01 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

g

Dosing volume
(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4059 mL 12.0294 mL 24.0587 mL 60.1468 mL
5 mM 0.4812 mL 2.4059 mL 4.8117 mL 12.0294 mL
10 mM 0.2406 mL 1.2029 mL 2.4059 mL 6.0147 mL
15 mM 0.1604 mL 0.8020 mL 1.6039 mL 4.0098 mL
20 mM 0.1203 mL 0.6015 mL 1.2029 mL 3.0073 mL
25 mM 0.0962 mL 0.4812 mL 0.9623 mL 2.4059 mL
30 mM 0.0802 mL 0.4010 mL 0.8020 mL 2.0049 mL
40 mM 0.0601 mL 0.3007 mL 0.6015 mL 1.5037 mL
50 mM 0.0481 mL 0.2406 mL 0.4812 mL 1.2029 mL
60 mM 0.0401 mL 0.2005 mL 0.4010 mL 1.0024 mL
80 mM 0.0301 mL 0.1504 mL 0.3007 mL 0.7518 mL
100 mM 0.0241 mL 0.1203 mL 0.2406 mL 0.6015 mL
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Hupehenine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Hupehenine98243-57-3α-synucleinReactive Oxygen Species (ROS)ApoptosisAutophagyParasiteα-SynSH-SY5Yisosteroidal alkaloidF. hupehensisParkinson's diseaseLeishmania donovaniInhibitorinhibitorinhibit

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