2. Protein Tyrosine Kinase/RTK JAK/STAT Signaling Cell Cycle/DNA Damage PI3K/Akt/mTOR
  3. EGFR PERK Akt
  4. GC1118

GC1118 (GC-1118A) is a fully human anti-EGFR monoclonal antibody with binding affinity of 0.16 nM (KD) to EGFR. GC1118 displays potent inhibitory effects on high- and low-affinity EGFR ligand-induced signaling. GC1118 shows potent anti proliferative activity in KRAS wild-type and KRAS mutant cells. GC1118 can reach the tumor by crossing both BBB (blood-brain barrier) and BTB (brain-tumor barrier) and shows superior anti-tumor effects in various mice xenograft models. GC1118 can be used for the researches of cancer, such as colorectal cancer.

For research use only. We do not sell to patients.

GC1118

GC1118 Chemical Structure

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GC1118 Related Antibodies

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Description

GC1118 (GC-1118A) is a fully human anti-EGFR monoclonal antibody with binding affinity of 0.16 nM (KD) to EGFR. GC1118 displays potent inhibitory effects on high- and low-affinity EGFR ligand-induced signaling. GC1118 shows potent anti proliferative activity in KRAS wild-type and KRAS mutant cells. GC1118 can reach the tumor by crossing both BBB (blood-brain barrier) and BTB (brain-tumor barrier) and shows superior anti-tumor effects in various mice xenograft models. GC1118 can be used for the researches of cancer, such as colorectal cancer[1][2][3][4].

Species Reactivity

Human
IC50 & Target

EGFR
In Vitro

GC1118 (GC-1118A) (1 μg/mL, 24-120 h) shows potent anti proliferative activity in KRAS wild-type and KRAS mutant cells[1][4].

GC1118 (100 µM, 6 days) suppresses cell growth in PDCs of G096 and G022[2].

GC1118 (0.1-50 μg/mL, 2 h) blocks EGFR high- and low-affinity ligand-induced EGFR signaling in HCT8 cells[3].

GC1118 (0.005-100 μg/mL, 3 days) inhibits high- and low-affinity ligand-induced proliferation in HCT8 cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

GC1118 Related Antibodies

Cell Proliferation Assay[1]

Cell Line: SNU-1, SNU-5, SNU-16, SNU-216, SNU-484, SNU-601, SNU-620, SNU-638, SNU-668, SNU-719, AGS, MKN-45 and NCI-N87 cells
Concentration: 0, 0.001, 0.01, 0.1, 1, 10, 100 and 1000 µg/ mL
Incubation Time: 72 h
Result: Suppressed cell growth, particularly in the MKN-45 cell line.

Cell Proliferation Assay[1]

Cell Line: SNU-484, SNU-601, SNU-719 and MKN-45 cells
Concentration: 1 µg/mL
Incubation Time: 24, 72, 120 h
Result: Showed more obviously anti-growth inhibitory effect in KRAS wild-type cell lines, SNU-719 and MKN-45, but also in KRAS mutant SNU-601 cells.

Western Blot Analysis[3]

Cell Line: HCT8 cells
Concentration: 0.1, 0.5, 5 and 50 μg/mL
Incubation Time: 2 h, stimulated with EGFR ligands (250 ng/mL EGF, HB-EGF, BTC, and TGF-α; 300 ng/mL AREG; 500 ng/mL EREG)
Result: Inhibited the high- and low-affinity ligand-induced Y1068 phosphorylation, Akt and Erk levels.
In Vivo

GC1118 (GC-1118A) (1 mg/kg, i.p., twice a week for 5 weeks) significantly suppresses xenograft tumor growth in AGS xenograft mice models[1].

GC1118 (50 mg/kg, i.p., twice a week) shows superior antitumor activity in patient-derived GBM xenograft models[2].

GC1118 (1 mg/mouse, i.p., twice a week for 5 weeks) significantly suppresses the tumor growth in HCT8, Lovo, HCT15, LS174T, LS513, and SW48 xenograft mice models[3].

GC1118 (1 mg/mouse, i.p., twice a week for 52 days) had moderate antitumor effects in CRC-024T patient-derived xenograft mice models[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: AGS xenograft mice models (athymic nude mice, female, 6 weeks, tumor volume of 200 mm3)[1]
Dosage: 1 mg/kg
Administration: Intraperitoneally injection, twice a week for 5 weeks
Result: Showed a significant suppression of the tumor growth.
Animal Model: Patient-derived GBM xenograft models (BALB/c nude mice, female, 6-8 weeks, tumor volume of 150-200 mm3)[2]
Dosage: 50 mg/kg
Administration: Intraperitoneal injection; twice a week until euthanasia
Result: Exerted anti-tumor effects in eight PDXs (G022, G677, B802, G608, G542, G096, G698, and G500).
Showed significantly better survival outcome than the control group.
Showed a significant amount accumulated in the tumor core.
Significantly increased tumor cell apoptosis and reduced microvascular density (MVD).
Animal Model: HCT8, Lovo, HCT15, LS174T, LS513, and SW48 xenograft mice models (athymic nude mice, 7-8 weeks, tumor volume of 200 mm3)[3]
Dosage: 1 mg/mouse
Administration: Intraperitoneally injection, twice a week for 5 weeks
Result: Significantly suppressed the tumor growth.
Animal Model: CRC-024T patient-derived xenograft mice models (BALB/c-nude mice, female, 6-8 weeks, tumor volume of 200-250 mm3)[4]
Dosage: 1 mg/mouse
Administration: Intraperitoneally injection, twice a week for 52 days
Result: Had moderate antitumor effects and no reduction in body weight.
Downregulated the levels of AKT and ERK1/2.
Gene ID

1956  [NCBI]

Accession

P00533-1
Target

EGFR
Application

ELISA, FACS, Functional assay
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
SMILES

N/A
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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GC1118 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

GC1118GC-1118AGC 1118GC-1118EGFRPERKAktEpidermal growth factor receptorErbB-1HER1Protein kinase R-like endoplasmic reticulum kinasePKR-like endoplasmic reticulum kinasePKBProtein kinase BCancerERKMKN-45 cellsHCT8 cellsPDXsInhibitorinhibitorinhibit

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