Flavone (Synonyms: 2-Phenyl-4-chromone)

Name: Flavone
Brand: MedChemExpress
SKU: HY-N2424
Rating: 4.5 (1 reviews)

Cat. No.: HY-N2424 Purity: 99.85%: Data Sheet
SDS

COA
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Flavone is an anti-tumor compound that targets cell cycle regulatory proteins (such as cyclin B1) and apoptosis-related factors (such as p21_waf1, PIG3). Flavone selectively induces mitochondrial-mediated apoptosis pathways in tumor cells, inhibits cyclin B1 protein expression, upregulates p21_waf1, and activates p63/p73 proteins. Flavone has immunomodulatory functions that enhance natural killer cell (NK cell) activity and lymphocyte proliferation. Flavone is used in cancer research, especially for its inhibitory potential in solid tumor models such as esophageal cancer and liver cancer.

For research use only. We do not sell to patients.

Flavone Chemical Structure

CAS No. : 525-82-6

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

Other Forms of Flavone:

Flavone (Standard) Get quote

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•Related Screening Libraries:

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View All CDK Isoform Specific Products:

View All Isoforms

CDK1 CDK2 CDK3 CDK4 CDK5 CDK6 CDK7 CDK8 CDK9 CDK11 CDK12 CDK13 CDK14 CDK16 CDK19 CDC CLK Pho85

View All Caspase Isoform Specific Products:

View All Isoforms

Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

CDK1/cyclinB1

Caspase 3

Caspase-9

Cellular Effect

Cell Line	Type	Value	Description	References
BALB/3T3	IC₅₀	287.1 μM Compound: 1	Growth inhibition of BALB/c mouse cloned 3T3/A31 cells after 72 hrs by nigrosin assay Growth inhibition of BALB/c mouse cloned 3T3/A31 cells after 72 hrs by nigrosin assay	[PMID: 10096863]
Caco-2	IC₅₀	21.01 μM Compound: 24	Cytotoxicity in human Caco2 cells after 72 hrs by MTT assay Cytotoxicity in human Caco2 cells after 72 hrs by MTT assay	[PMID: 28793973]
H9	EC₅₀	50 μM Compound: 9	Antiviral activity against HIV1 3B infected in human H9 cells assessed as inhibition of viral replication after 3 days by p24 antigen capture assay Antiviral activity against HIV1 3B infected in human H9 cells assessed as inhibition of viral replication after 3 days by p24 antigen capture assay	[PMID: 8158164]
H9	IC₅₀	68 μM Compound: 9	Cytotoxicity against human H9 cells after 3 days Cytotoxicity against human H9 cells after 3 days	[PMID: 8158164]
HepG2	GI₅₀	50.6 μM Compound: 1a	Cytotoxicity against Homo sapiens (human) HepG2 cells after 48 hr by SRB assay Cytotoxicity against Homo sapiens (human) HepG2 cells after 48 hr by SRB assay	10.1007/s00044-012-0423-1
MCF7	IC₅₀	0.3 μM Compound: Flavone	Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 6 days by SRB assay Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 6 days by SRB assay	[PMID: 33257172]
MCF7	IC₅₀	26 μM Compound: 7	Inhibition of BCRP expressed in MCF-7 MX cells using Hoechst 33342 staining Inhibition of BCRP expressed in MCF-7 MX cells using Hoechst 33342 staining	[PMID: 21354800]
MCF7	GI₅₀	69.3 μM Compound: 1a	Cytotoxicity against Homo sapiens (human) MCF7 cells after 48 hr by SRB assay Cytotoxicity against Homo sapiens (human) MCF7 cells after 48 hr by SRB assay	10.1007/s00044-012-0423-1
MDCK	IC₅₀	17 μM Compound: 7	Inhibition of BCRP expressed in MDCK cells using Hoechst 33342 staining Inhibition of BCRP expressed in MDCK cells using Hoechst 33342 staining	[PMID: 21354800]
Monocyte	IC₅₀	166 μM Compound: flavone	Inhibition of TNFalpha expression in LPS-stimulated human monocytes treated 30 mins before LPS challenge measured after 14 hrs by ELISA Inhibition of TNFalpha expression in LPS-stimulated human monocytes treated 30 mins before LPS challenge measured after 14 hrs by ELISA	[PMID: 10096854]
Neutrophil	IC₅₀	57 μM Compound: 1a	Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of superoxide anion radical-induced lucigenin oxidation incubated for 5 mins prior to PMA challenge by chemiluminescence assay Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of superoxide anion radical-induced lucigenin oxidation incubated for 5 mins prior to PMA challenge by chemiluminescence assay	[PMID: 23871908]
Neutrophil	IC₅₀	64.9 μM Compound: 1a	Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of HOCl-induced oxidation of APF after 6 mins by fluorescence assay Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of HOCl-induced oxidation of APF after 6 mins by fluorescence assay	[PMID: 23871908]

In Vitro

Flavone (10-80 μM; 24-72 h) inhibits the cell viability of the human esophageal squamous cell carcinoma cell line KYSE-510 in a dose- and time-dependent manner^[2].
Flavone (80 μM; 24 h) induces G2/M arrest in KYSE-510 cells, accompanied by upregulation of p21_waf1 mRNA and protein expression and downregulation of cyclin B1 expression^[2].
Flavone (80 μM; 24 h) triggeres apoptosis in KYSE-510 cells, as evidenced by DNA fragmentation, increases number of Annexin V/PI double-stained positive cells, and activation of caspase-9 and caspase-3^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	Human esophageal squamous cell carcinoma cell line (KYSE-510)
Concentration:	10, 20, 40, 80 μM
Incubation Time:	24, 48, 72 h
Result:	Significantly reduced cell viability in a dose- and time-dependent manner.

In Vivo

Flavone (25, 50, 100 mg/kg; intraperitoneal injection; once a day; for 10 consecutive days) significantly inhibits tumor growth in mouse S180 sarcoma and H22 liver cancer xenograft models, and enhances immune organ index and NK cell activity^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Kunming mice (male and female, 18-21 g) with S180 sarcoma or H22 hepatoma xenografts^[3]
Dosage:	25, 50, 100 mg/kg
Administration:	Intraperitoneal injection, once daily for 10 days
Result:	Significantly reduced tumor weight in both models compared to the negative control. For S180 sarcoma, tumor weights were 1.38 g (25 mg/kg), 1.07 g (50 mg/kg), and 1.25 g (100 mg/kg) vs. 2.12 g in the control, with inhibition rates of 34.91%, 50.47%, and 41.04%, respectively. For H22 hepatoma, tumor weights were 1.47 g (25 mg/kg), 1.01 g (50 mg/kg), and 1.30 g (100 mg/kg) vs. 2.16 g in the control, with inhibition rates of 31.94%, 53.24%, and 39.81%, respectively. The middle dose (50 mg/kg) showed the strongest effect, comparable to the positive control (cyclophosphamide, CTX).

Molecular Weight

222.24

Formula

C₁₅H₁₀O₂

CAS No.

525-82-6

Appearance

Solid

Color

White to off-white

SMILES

O=C1C=C(C2=CC=CC=C2)OC3=CC=CC=C13

Structure Classification

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (449.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.4996 mL	22.4982 mL	44.9964 mL
5 mM	0.8999 mL	4.4996 mL	8.9993 mL
10 mM	0.4500 mL	2.2498 mL	4.4996 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 4.55 mg/mL (20.47 mM); Clear solution

This protocol yields a clear solution of ≥ 4.55 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (45.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (11.25 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (11.25 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.85%

Select Batch:

Data Sheet (277 KB) SDS (393 KB)

COA (250 KB) HNMR (252 KB) LCMS (98 KB)

Handling Instructions (2659 KB)

References

[1]. Chirumbolo S. The role of quercetin, flavonols and flavones in modulating inflammatory cell function. Inflamm Allergy Drug Targets. 2010 Sep;9(4):263-85. [Content Brief]

[2]. Zhang Q, et al. Cytotoxicity of flavones and flavonols to a human esophageal squamous cell carcinoma cell line (KYSE-510) by induction of G2/M arrest and apoptosis. Toxicol In Vitro. 2009 Aug;23(5):797-807. [Content Brief]

[3]. Liu S, et al. Tumor inhibition and improved immunity in mice treated with flavone from Cirsium japonicum DC. Int Immunopharmacol. 2006 Sep;6(9):1387-93. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.4996 mL	22.4982 mL	44.9964 mL	112.4910 mL
	5 mM	0.8999 mL	4.4996 mL	8.9993 mL	22.4982 mL
	10 mM	0.4500 mL	2.2498 mL	4.4996 mL	11.2491 mL
	15 mM	0.3000 mL	1.4999 mL	2.9998 mL	7.4994 mL
	20 mM	0.2250 mL	1.1249 mL	2.2498 mL	5.6245 mL
	25 mM	0.1800 mL	0.8999 mL	1.7999 mL	4.4996 mL
	30 mM	0.1500 mL	0.7499 mL	1.4999 mL	3.7497 mL
	40 mM	0.1125 mL	0.5625 mL	1.1249 mL	2.8123 mL
	50 mM	0.0900 mL	0.4500 mL	0.8999 mL	2.2498 mL
	60 mM	0.0750 mL	0.3750 mL	0.7499 mL	1.8748 mL
	80 mM	0.0562 mL	0.2812 mL	0.5625 mL	1.4061 mL
	100 mM	0.0450 mL	0.2250 mL	0.4500 mL	1.1249 mL

Flavone Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Flavone525-82-62-Phenyl-4-chromoneCDKCaspaseApoptosisCyclin dependent kinaseInhibitorinhibitorinhibit

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Flavone (Synonyms: 2-Phenyl-4-chromone)

Customer Review

Other Forms of Flavone:

Flavone Related Antibodies

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View All CDK Isoform Specific Products:

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Biological Activity

Purity & Documentation

References

Customer Review

Flavone Related Antibodies

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Complete Stock Solution Preparation Table

Flavone Related Classifications

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