2. MAPK/ERK Pathway Apoptosis Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. Mixed Lineage Kinase Necroptosis Pyroptosis Caspase NOD-like Receptor (NLR) Keap1-Nrf2 TNF Receptor Interleukin Related NO Synthase Heme Oxygenase (HO)
  4. (E/Z)-Necrosulfonamide

(E/Z)-Necrosulfonamide is a racemic compound of Necrosulfonamide (HY-100573). Necrosulfonamide is a MLKL and Gasdermin D (GSDMD) inhibitor, capable of separately inhibiting necroptosis and pyroptosis of cells. Necrosulfonamide does not affect the activation of upstream signals, but specifically inhibits the downstream executor oligomerization step. Necrosulfonamide reduces the expression of the key kinases NLRP3 and caspase-1 involved in necroptosis and pyroptosis, activate the Nrf2 pathway and the downstream antioxidant enzymes, and also downregulates a variety of inflammatory factors. Necrosulfonamide plays significant roles in various diseases such as neurodegenerative diseases (such as Parkinson’s disease), tissue damage and ischemia-reperfusion injury, inflammatory bowel disease, osteoarthritis and fracture repair, and hair loss by regulating two important programmed necrosis pathways.

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(E/Z)-Necrosulfonamide

(E/Z)-Necrosulfonamide Chemical Structure

CAS No. : 432531-71-0

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Based on 2 publication(s) in Google Scholar

Other Forms of (E/Z)-Necrosulfonamide:

(E/Z)-Necrosulfonamide Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE (E/Z)-Necrosulfonamide

IF

    (E/Z)-Necrosulfonamide purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2020 Jul;24(14):8078-8090.  [Abstract]

    Results of PI and Hoechst 33 258 staining: Aβ1‐42 intervention increases the number of positive‐staining cells, while NSA intervention significantly decreases the number of positive-staining cells, and NSA could attenuated the opening degree of membrane pores.

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    Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

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    NLRP3 NLRP1 NOD1 NOD2

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    TNFRSF1A/CD120a TNFRSF3/CD18 TNFRSF4 TNFRSF5/CD40 TNFRSF6/Fas/CD95 TNFRSF7/CD27 TNFRSF8/CD30 TNFRSF9/4-1BB/CD137 TNFRSF12A/TWEAK TNFRSF16/NGF Receptor/CD271 TNFRSF18/GITR/CD357

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    IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22

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    nNOS iNOS eNOS

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    HO-1 HO-2

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    • Customer Review

    Description

    (E/Z)-Necrosulfonamide is a racemic compound of Necrosulfonamide (HY-100573). Necrosulfonamide is a MLKL and Gasdermin D (GSDMD) inhibitor, capable of separately inhibiting necroptosis and pyroptosis of cells. Necrosulfonamide does not affect the activation of upstream signals, but specifically inhibits the downstream executor oligomerization step. Necrosulfonamide reduces the expression of the key kinases NLRP3 and caspase-1 involved in necroptosis and pyroptosis, activate the Nrf2 pathway and the downstream antioxidant enzymes, and also downregulates a variety of inflammatory factors. Necrosulfonamide plays significant roles in various diseases such as neurodegenerative diseases (such as Parkinson’s disease), tissue damage and ischemia-reperfusion injury, inflammatory bowel disease, osteoarthritis and fracture repair, and hair loss by regulating two important programmed necrosis pathways.

    Cellular Effect
    Cell Line Type Value Description References
    HT-29 IC50
    < 200 nM
    Compound: 66; NSA
    Anti-neprotic activity in human HT-29 cells assessed as reduction in TSZ-induced necroptosis incubated for 24 hrs by cell titer glo-based luminescence assay
    Anti-neprotic activity in human HT-29 cells assessed as reduction in TSZ-induced necroptosis incubated for 24 hrs by cell titer glo-based luminescence assay
    		[PMID: 31622096]
    Molecular Weight

    461.47

    Formula

    C18H15N5O6S2
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C(/C=C/C1=CC=C(S1)[N+]([O-])=O)NC2=CC=C(C=C2)S(=O)(NC3=NC=CN=C3OC)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (216.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.1670 mL 10.8349 mL 21.6699 mL
    5 mM 0.4334 mL 2.1670 mL 4.3340 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.1670 mL 10.8349 mL 21.6699 mL 54.1747 mL
    5 mM 0.4334 mL 2.1670 mL 4.3340 mL 10.8349 mL
    10 mM 0.2167 mL 1.0835 mL 2.1670 mL 5.4175 mL
    15 mM 0.1445 mL 0.7223 mL 1.4447 mL 3.6116 mL
    20 mM 0.1083 mL 0.5417 mL 1.0835 mL 2.7087 mL
    25 mM 0.0867 mL 0.4334 mL 0.8668 mL 2.1670 mL
    30 mM 0.0722 mL 0.3612 mL 0.7223 mL 1.8058 mL
    40 mM 0.0542 mL 0.2709 mL 0.5417 mL 1.3544 mL
    50 mM 0.0433 mL 0.2167 mL 0.4334 mL 1.0835 mL
    60 mM 0.0361 mL 0.1806 mL 0.3612 mL 0.9029 mL
    80 mM 0.0271 mL 0.1354 mL 0.2709 mL 0.6772 mL
    100 mM 0.0217 mL 0.1083 mL 0.2167 mL 0.5417 mL
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    (E/Z)-Necrosulfonamide Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    (E/Z)-Necrosulfonamide432531-71-0Mixed Lineage KinaseNecroptosisPyroptosisCaspaseNOD-like Receptor (NLR)Keap1-Nrf2TNF ReceptorInterleukin RelatedNO SynthaseHeme Oxygenase (HO)MLKsTumor Necrosis Factor ReceptorTNFRILNitric oxide synthasesNOSHeme OxygenaseInhibitorinhibitorinhibit

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