1. NF-κB MAPK/ERK Pathway Apoptosis
  2. NF-κB p38 MAPK Bcl-2 Family Caspase Apoptosis
  3. DNH28

DNH28 is a potent NF-κB and MAPK inhibitor with an IC50 of 0.93 μM against HepG2 cells. DNH28 promotes apoptosis by down-regulating the expression of Bcl-2, up-regulating the expression of BAX and C-caspase-3. DNH28 blocks the cell cycle and inhibits migration. DNH28 can be used for the study of hepatocellular carcinoma (HCC).

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DNH28

DNH28 Chemical Structure

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Description

DNH28 is a potent NF-κB and MAPK inhibitor with an IC50 of 0.93 μM against HepG2 cells. DNH28 promotes apoptosis by down-regulating the expression of Bcl-2, up-regulating the expression of BAX and C-caspase-3. DNH28 blocks the cell cycle and inhibits migration. DNH28 can be used for the study of hepatocellular carcinoma (HCC)[1].

IC50 & Target[1]

Caspase 3

 

Bax

 

Bcl-2

 

NF-κB

 

In Vitro

DNH28 (Compound 28) exhibits lower toxicity toward THLE-3 cells (IC50 = 18.16 μM)[1].
DNH28 (0.46-1.84 μM, 24 h) inhibits HepG2 cells proliferation by inducing apoptosis and dose-dependently inhibits HepG2 cells in the G2/M phase[1].
DNH28 (0.46-1.84 μM, 24 h) inhibits HepG2 cell migration dose-dependently, and it completely inhibits the migration ability at a concentration of 1.84 μM[1].
DNH28 (1.84 μM, 24 h) effectively inhibits the nuclear translocation of p65 induced by TNF-α[1].
DNH28 (0.46-1.84 μM, 24 h) exerts anti-HCC effects by inhibiting the activation of NF-κB and MAPK signaling pathways[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis[1]

Cell Line: HepG2 cells
Concentration: 0.46, 0.92 and 1.84 μM
Incubation Time: 24 h
Result: Induced apoptosis dose-dependently.
Reached 45.90% of apoptotic cells at 1.84 μM (compared with 14.57% in the control group).

Cell Cycle Analysis[1]

Cell Line: HepG2 cells
Concentration: 0.46, 0.92 and 1.84 μM
Incubation Time: 24 h
Result: Reached 76.22% G2/M phase ratio at 1.84 μM.

Cell Migration Assay [1]

Cell Line: HepG2 cells
Concentration: 0.46, 0.92 and 1.84 μM
Incubation Time: 24 h
Result: Partially suppressed cell migration, whereas at 1.84 μM, the scratch width remained unchanged.

RT-PCR[1]

Cell Line: HepG2 cells
Concentration: 0.46, 0.92 and 1.84 μM
Incubation Time: 24 h
Result: Increase the expression of pro-apoptotic mRNA level BAX and C-caspase-3.
Reduce the expression of the anti-apoptotic mRNA level BCL-2.

Western Blot Analysis[1]

Cell Line: HepG2 cells
Concentration: 0.46, 0.92 and 1.84 μM
Incubation Time: 24 h
Result: Exhibited dose-dependent inhibition of the phosphorylation of p65, IκB-α, p38 and ERK.
Significantly down-regulated the activation of the NF-κB and MAPK signaling pathways
Molecular Weight

436.90

Formula

C23H24ClF3N2O

SMILES

CCN1CCN(C2=CC(CC/C(C3=O)=C\C4=CC=C(F)C(F)=C4F)=C3C=C2)CC1.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
DNH28
Cat. No.:
HY-175037
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