DNA polymerase-IN-7 

DNA polymerase-IN-7 is a noncompetitive inhibition of DNA Polymerase β with an IC₅₀ of 112 nM and a K_i of 35 nM. DNA polymerase-IN-7 can inhibit DNA pol β lyase activity and the binding of the lyase domain of Pol β to DNA. DNA polymerase-IN-7 can be used for the research of cancer, such as cervical cancer^[1].

DNA polymerase-IN-7 (Compound 28) (0.1-1 μM) inhibits dGTP incorporation activity of DNA polymerase β (Pol β) and inhibits the lyase activity of Pol β^[1].
DNA polymerase-IN-7 (0.35-2 μM) exerts little effect on the binding of Pol β to DNA, but inhibits the binding of the lyase domain of Pol β to DNA^[1].
DNA polymerase-IN-7 (0.1-1 μM) exhibits selectivity for X-family polymerases, with inhibitory rate of 50% for Pol β and 41% for Pol λ at 0.1 μM, and almost 100% at 1 μM^[1].
DNA polymerase-IN-7 (0-100 μM, 24 h) shows no obvious cytotoxicity, maintaining cell viability at 95-100% in HeLa cells^[1].
DNA polymerase-IN-7 (25 μM, 24 h) shows intracellular concentration reaching 4.0 μM in HeLa cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

901.90

C₄₇H₄₈N₇O₁₀P

O=C(C1=CC=C(C2=CC=C(C3=CC=C(C4=CC=C(O)C=C4)C=C3)C=C2)C=C1)N[C@H]5C[C@H](N6C=C(CCCNC(C7=CN8C(C=C(C)C=C8)=N7)=O)C(NC6=O)=O)O[C@@H]5COP([O-])(OC)=O.[NH4+]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Howlader AH, et al. Noncompetitive Inhibition of DNA Polymerase β by a Nonnative Nucleotide. J Org Chem. 2025 Sep 19;90(37):12849-12859.  [Content Brief]