Direct Blue 86  (Synonyms: Solvent Blue 38)

Direct Blue 86 (Solvent Blue 38) is a myelin-sheath stain, commonly utilized in microscopy to detect demyelination in the central nervous system. Direct Blue 86 also is a dye with various applications including as a commercial dye in the printing of cotton and mucilage glue fabrics^[1].

Guidelines (The following is our recommended protocol, which is provided as a guide only and should be modified to suit your specific needs).
1. Preparation of Dye Stock Solution^[1]
1) Weigh an appropriate amount of Direct Blue-86.
2) Dissolve it in 100 mL of distilled water.
3) Transfer the solution to a 1000-mL volumetric flask and make up to the mark with distilled water to obtain a stock solution with a concentration of 1.0 g/L.
2. Preparation of Dye Working Solutions
1) Take aliquots of the 1.0 g/L stock solution.
2) Dilute them with distilled water to prepare working solutions with concentrations ranging from 5 to 100 mg/L according to the experimental requirements.
3. Specific Adsorption Steps (Effect of pH on Adsorption)
1) Prepare a series of solutions with initial pH values adjusted from 1 to 9 using 0.1 M HCl or 0.1 M NaOH.
2) Add 6 g/L of activated carbon prepared from orange peel (COP) to each solution containing 100 mg/L of Direct Blue-86.
3) Shake the suspensions at room temperature 25℃ with an agitation speed of 200 rpm for 180 min, which is the minimum contact time required to reach equilibrium.
4) Determine the amount of Direct Blue-86 adsorbed.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

780.16

C₃₂H₁₄CuN₈O₆S₂.2Na

1330-38-7

Solid

Dark purple to black

CS(=O)(O[Na])=O.CS(=O)(O[Na])=O.C12=C(N=C3C(C=CC=C4)=C4C5=[N]3[Cu+2]67[N-]8C(C(C=CC=C9)=C9C8=NC%10=[N]7C(C%11=C%10C=CC=C%11)=N%12)=N5)[N-]6C%12=C1C=CC=C2

Room temperature in continental US; may vary elsewhere.

RT, stored under nitrogen, away from moisture

• [1]. Ahmed El Nemr, et al. Removal of direct blue-86 from aqueous solution by new activated carbon developed from orange peel. J Hazard Mater. 2009 Jan 15;161(1):102-10.  [Content Brief]