2. Membrane Transporter/Ion Channel
  3. Sodium Channel
  4. DAPI

DAPI  (Synonyms: 4',6-Diamidino-2-phenylindole)

Cat. No.: HY-D2868 Purity: 98.01%
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DAPI (Compound 3) is an acid-sensing ion channel 3 (ASIC3) inhibitor. DAPI binds to ASIC3 and blocks the channel function. DAPI can be used in the study of chronic pain treatment.

For research use only. We do not sell to patients.

DAPI

DAPI Chemical Structure

CAS No. : 47165-04-8

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Customer Review

Based on 105 publication(s) in Google Scholar

Other Forms of DAPI:

DAPI Related Antibodies

Top Publications Citing Use of Products

100 Publications Citing Use of MCE DAPI

IF

    DAPI purchased from MedChemExpress. Usage Cited in: Pharmaceuticals (Basel). 2022 Jun 6;15(6):721.

    The nuclei are counterstained with DAPI (10 μM) in the dark for 10 min at room temperature. Fluorescence is monitored using confocal microscope, and foci of phospho-H2A.X was quantified using ImageJ software.

    DAPI purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2021 Jul;42(7):1111-1123.  [Abstract]

    Mice intestine tissues sections were incubated with a TUNEL reaction mixture for 1 h at 37 °C in the dark. Next, the sections are stained with 0.1 μg/mL DAPI.

    DAPI purchased from MedChemExpress. Usage Cited in: Oncotargets Ther. 2020 Oct 6;13:9941-9952.  [Abstract]

    Human GC MGC-803 and NCI-N87 cells are seeded into 24-well plates with glass slide for 24h in RPMI-1640 medium. And 0.1% DAPI staining is performed against light for 30 minutes.

    DAPI purchased from MedChemExpress. Usage Cited in: Acta Biomater. 2019 Sep 15:96:468-479.  [Abstract]

    CLSM observations of tested Cy5-nanocomplexes incubated with RAW 264.7 macrophages show the fluorescent distributions of DAPI, nanocomplexes, and ROS (DCFH-DA) in the presence and absence NIR treatment.

    DAPI purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2020 Mar;68:153146.  [Abstract]

    MC3T3-E1 cells incubated with 1 μg/ml DAPI dihydrochloride for 5 min and observed them under a fluorescence microscope. Effects of sweroside on ER-α and GPR30 expressions by immunofluorescence, white and yellow arrows mean target protein distribution in the cell nuclei and membrane, respectively.

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    • Biological Activity

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    Description

    DAPI (Compound 3) is an acid-sensing ion channel 3 (ASIC3) inhibitor. DAPI binds to ASIC3 and blocks the channel function. DAPI can be used in the study of chronic pain treatment[1].

    Molecular Weight

    277.32

    Formula

    C16H15N5
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    N=C(C1=CC=C(C2=CC3=CC=C(C(N)=N)C=C3N2)C=C1)N
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 9.09 mg/mL (32.78 mM; ultrasonic and adjust pH to 3 with 1 M HCL; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.6059 mL 18.0297 mL 36.0594 mL
    5 mM 0.7212 mL 3.6059 mL 7.2119 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.6059 mL 18.0297 mL 36.0594 mL 90.1486 mL
    5 mM 0.7212 mL 3.6059 mL 7.2119 mL 18.0297 mL
    10 mM 0.3606 mL 1.8030 mL 3.6059 mL 9.0149 mL
    15 mM 0.2404 mL 1.2020 mL 2.4040 mL 6.0099 mL
    20 mM 0.1803 mL 0.9015 mL 1.8030 mL 4.5074 mL
    25 mM 0.1442 mL 0.7212 mL 1.4424 mL 3.6059 mL
    30 mM 0.1202 mL 0.6010 mL 1.2020 mL 3.0050 mL
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    DAPI Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    DAPI47165-04-84',6-Diamidino-2-phenylindoleSodium ChannelNa channelsNa+ channelsASIC3painSD ratsCFAInhibitorinhibitorinhibit

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