Crystal Violet (solution) 

Crystal Violet (solution), also known as Gentian violet, methyl violet 10B, is a triphenyl-methane, an alkaline dye that binds to DNA in the nucleus of a cell, staining it a deep purple. It is often used for Gram staining to classify bacteria, or for cell or histological staining^[1].
Solution Concentration: 5 mg/mL (0.5%)

General Protocol

1.Preparation of MitoSOX Red working solution
Dilute the 2 g Crystal Violet in 20 mL 95% ethanol andmixed with 80 ml 1% ammonium oxalate solution solution for 24 h to obtain working solution.
2.Sample processing
2.1 Paraffin section:
First dewaxing with xylene for 5-10 min, then with fresh xylene, then dewaxing with 100% ethanol for 5 min after dewaxing for 5-10 min. Then 90% ethanol was used for 2 min. At last, 70% ethanol was used for 2 min. DDH₂O 2 min.
2.2 Frozen section:
distilled water for 2 min.
2.3 Tissue culture cells:
fixed with 4% paraformaldehyde for more than 10 min. Wash with distilled water for 2 min, replace with fresh distilled water, and wash again for 2 min.
Note: Other fixatives can be selected according to specific experiments.
3.Crystal violet staining
3.1 After the sample to be tested is treated with the above method, the crystal violet dyeing solution is directly added and dyed for 10 minutes at room temperature (the dyeing solution needs to cover the sample, and the specific dyeing time can be adjusted according to the required dyeing results).
3.2 Wash well in distilled water.
3.3 Dry at room temperature.
3.4 Observe and photograph by light microscope.

Storage
4°C, 2 year
Protect from light

Precautions
1. It is recommended to take 1-2 samples for pre-test when using this product for the first time.
2. This product is for R&D use only, not for drug, household, or other uses.
3. For your safety and health, please wear a lab coat and disposable gloves to operate.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

407.99

C₂₅H₃₀ClN₃

548-62-9

C/[N+](C)=C1C=C/C(C=C/1)=C(C2=CC=C(N(C)C)C=C2)\C3=CC=C(N(C)C)C=C3.[Cl-]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Nagayama A. Inactivation of influenza A virus by gentian violet (GV) and GV-dyed cotton cloth, and bactericidal activities of these agents. J Infect Chemother. 2006 Apr;12(2):73-9.  [Content Brief]

  [2]. Beveridge, T.J. and S. Schultze-Lam, The response of selected members of the archaea to the gram stain. Microbiology, 1996. 142 ( Pt 10): p. 2887-95.  [Content Brief]

  [3]. Coico, R., Gram staining. Curr Protoc Microbiol, 2005. Appendix 3: p. Appendix 3C.  [Content Brief]

  [4]. Bil, J., et al., Statins potentiate cytostatic/cytotoxic activity of sorafenib but not sunitinib against tumor cell lines in vitro. Cancer Lett, 2010. 288(1): p. 57-67.  [Content Brief]

  [5]. Xiao X, et al. Analysis of trace malachite green, crystal violet, and their metabolites in zebrafish by surface-coated probe nanoelectrospray ionization mass spectrometry. Talanta. 2020 Sep 1;217:121064.  [Content Brief]