2. NF-κB Apoptosis Metabolic Enzyme/Protease Immunology/Inflammation
  3. Keap1-Nrf2 Apoptosis Reactive Oxygen Species (ROS) NF-κB
  4. CPUY192018

CPUY192018 is a potent inhibitor of the Keap1-Nrf2 protein-protein interaction, with an IC50 of 0.63 µM. CPUY192018 exhibits anti-inflammatory and antioxidant activities. CPUY192018 can activate the Nrf2-dependent antioxidant pathway and inhibit the NF-κB-related inflammatory response. CPUY192018 can be used in the research of inflammation-related diseases.

For research use only. We do not sell to patients.

CPUY192018 Chemical Structure

CPUY192018 Chemical Structure

CAS No. : 1567836-15-0

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
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10 mg In-stock
25 mg In-stock
50 mg In-stock
100 mg In-stock
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Based on 1 publication(s) in Google Scholar

CPUY192018 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE CPUY192018

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

CPUY192018 is a potent inhibitor of the Keap1-Nrf2 protein-protein interaction, with an IC50 of 0.63 µM. CPUY192018 exhibits anti-inflammatory and antioxidant activities. CPUY192018 can activate the Nrf2-dependent antioxidant pathway and inhibit the NF-κB-related inflammatory response. CPUY192018 can be used in the research of inflammation-related diseases[1][2].

In Vitro

CPUY192018 (0-10 μM; 0-24 h) can significantly increase the nuclear translocation and level of Nrf2, as well as the expression of Nrf2 downstream proteins in HK-2 cells[1].
CPUY192018 (0-10 μM; 16-24 h) can inhibit ROS production, reduce MDA levels, increase the GSH/GSSG ratio, inhibit apoptosis, cytokine secretion, and the activation of the NF-κB signaling pathway in HK-2 cells treated with LPS (HY-D1056)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CPUY192018 Related Antibodies

Western Blot Analysis[1]

Cell Line: HK-2 cells treated with LPS (HY-D1056)
Concentration: 1 and 10μM
Incubation Time: Pretreated with 10 h, then co-incubation for 6 h
Result: Inhibited the phosphorylation levels of IKKβ, IκBα and NF-κB p65.
In Vivo

CPUY192018 (5-20 mg/kg; intraperitoneal injection; 8 weeks) has an ameliorating effect in a mouse model of chronic renal inflammation induced by LPS (HY-D1056)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female C57BL/6 mice aged 6-8 weeks old (18-20 g) treated LPS (HY-D1056)[1]
Dosage: 5 and 20 mg/kg
Administration: Intraperitoneal injection; 8 weeks
Result: Attenuated the body weight loss in mice.
Reduced histological disease scores.
Improved glomerular pathological changes, activated the Nrf2 pathway to enhance renal antioxidant capacity and inhibited the inflammatory response.
Molecular Weight

614.64

Formula

C28H26N2O10S2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(O)CN(C1=C2C=CC=CC2=C(N(S(=O)(C3=CC=C(OC)C=C3)=O)CC(O)=O)C=C1)S(=O)(C4=CC=C(OC)C=C4)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (406.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6270 mL 8.1348 mL 16.2697 mL
5 mM 0.3254 mL 1.6270 mL 3.2539 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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C2

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V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (3.38 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.08 mg/mL (3.38 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.6270 mL 8.1348 mL 16.2697 mL 40.6742 mL
5 mM 0.3254 mL 1.6270 mL 3.2539 mL 8.1348 mL
10 mM 0.1627 mL 0.8135 mL 1.6270 mL 4.0674 mL
15 mM 0.1085 mL 0.5423 mL 1.0846 mL 2.7116 mL
20 mM 0.0813 mL 0.4067 mL 0.8135 mL 2.0337 mL
25 mM 0.0651 mL 0.3254 mL 0.6508 mL 1.6270 mL
30 mM 0.0542 mL 0.2712 mL 0.5423 mL 1.3558 mL
40 mM 0.0407 mL 0.2034 mL 0.4067 mL 1.0169 mL
50 mM 0.0325 mL 0.1627 mL 0.3254 mL 0.8135 mL
60 mM 0.0271 mL 0.1356 mL 0.2712 mL 0.6779 mL
80 mM 0.0203 mL 0.1017 mL 0.2034 mL 0.5084 mL
100 mM 0.0163 mL 0.0813 mL 0.1627 mL 0.4067 mL
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CPUY192018 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CPUY1920181567836-15-0CPUY 192018CPUY-192018Keap1-Nrf2ApoptosisReactive Oxygen Species (ROS)NF-κBNuclear factor-κBNuclear factor-kappaBchronic renal inflammation modelHK-2 cellsInhibitorinhibitorinhibit

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