2. Immunology/Inflammation Apoptosis
  3. COX Bcl-2 Family Caspase
  4. COX-2-IN-59

COX-2-IN-59 is a potent and selective COX-2 inhibitor with an IC50 of 0.052 μM. COX-2-IN-59 exhibits >200-fold selectivity over COX-1 (IC50 = 11.16 μM). COX-2-IN-59 reduces COX-2 levels, induces cell cycle arrest, and triggers apoptosis by increasing Bax expression, decreasing Bcl-2 levels, and activating caspase-3. COX-2-IN-59 can be used for the research of colon cancer.

For research use only. We do not sell to patients.

COX-2-IN-59

COX-2-IN-59 Chemical Structure

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COX-2-IN-59 Related Antibodies

Top Publications Citing Use of Products

View All COX Isoform Specific Products:

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COX COX-1 COX-2 COX-3

View All Bcl-2 Family Isoform Specific Products:

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Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim BNIP3

View All Caspase Isoform Specific Products:

View All Isoforms
Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

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Description

COX-2-IN-59 is a potent and selective COX-2 inhibitor with an IC50 of 0.052 μM. COX-2-IN-59 exhibits >200-fold selectivity over COX-1 (IC50 = 11.16 μM). COX-2-IN-59 reduces COX-2 levels, induces cell cycle arrest, and triggers apoptosis by increasing Bax expression, decreasing Bcl-2 levels, and activating caspase-3. COX-2-IN-59 can be used for the research of colon cancer[1].

IC50 & Target[1]

COX-2

0.052 μM (IC50)

COX-1

11.16 μM (IC50)

In Vitro

COX-2-IN-59 (compound 5d) (12.5-100 μM; 24 h) inhibits A-549, HOS, MCF‐7, HT‐29, PC3, and Caco‐2 cell growth with IC50s of 4, 67.3, 30.5, 8.5, 29.1, and 2.6 μM, respectively[1].
COX-2-IN-59 (2.6 μM; 24 h) induces G1/S cell cycle arrest, and triggers apoptosis in Caco-2 cells by increasing Bax expression, decreasing Bcl-2 levels, and activating caspase-3[1].
COX-2-IN-59 (2.6 μM; 24 h) significantly reduces COX-2 levels to 3.858 ng/mL (0.494‐fold relative to control) in Caco-2 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

COX-2-IN-59 Related Antibodies

Cell Viability Assay[1]

Cell Line: A-549, HOS, MCF‐7, HT‐29, PC3, and Caco‐2 cells
Concentration: 12.5-100 μM
Incubation Time: 24 h
Result: Inhibited A-549, HOS, MCF‐7, HT‐29, PC3, and Caco‐2 cell growth with IC50s of 4, 67.3, 30.5, 8.5, 29.1, and 2.6 μM, respectively.

Apoptosis Analysis[1]

Cell Line: Caco-2
Concentration: 2.6 μM
Incubation Time: 24 h
Result: Demonstrated potent proapoptotic activity in Caco‐2 cells, inducing a nearly eightfold increase in total apoptosis (24.38% vs. 3.16% in controls). Induced a dramatic 63‐fold increase (15.86% vs. 0.25%) in late apoptosis, and an 11‐fold increase (4.85% vs. 0.42%) in early apoptosis.

Cell Cycle Analysis[1]

Cell Line: Caco-2
Concentration: 2.6 μM
Incubation Time: 24 h
Result: Showed a dramatic decrease in G2/M phase cells in the treated group (3.82%) compared to the control group (22.05%). Significantly increased cells in the G0/G1 phase (64.06% vs. 48.29% in control). Induced G1/S phase cell cycle arrest.

Western Blot Analysis[1]

Cell Line: Caco-2
Concentration: 2.6 μM
Incubation Time: 24 h
Result: Induced the expression of Bax while suppressing the expression of Bcl‐2, ultimately leading to an increased expression of caspase‐3.

ELISA Assay[1]

Cell Line: Caco-2
Concentration: 2.6 μM
Incubation Time: 24 h
Result: Significantly reduced COX-2 levels to 3.858 ng/mL (0.494‐fold relative to control).
Molecular Weight

405.42

Formula

C18H19N3O6S
SMILES

CC(NC1=CC=C(OCC(N/N=C/C2=CC=C(OS(=O)(C)=O)C=C2)=O)C=C1)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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COX-2-IN-59 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

COX-2-IN-59COXBcl-2 FamilyCaspaseCyclooxygenaseCOX-2,colon cancer,Caco-2Inhibitorinhibitorinhibit

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