2. JAK/STAT Signaling Protein Tyrosine Kinase/RTK Metabolic Enzyme/Protease Cell Cycle/DNA Damage Vitamin D Related/Nuclear Receptor PI3K/Akt/mTOR Epigenetics
  3. EGFR Cytochrome P450 Fatty Acid Synthase (FASN) Acetyl-CoA Carboxylase HMG-CoA Reductase (HMGCR) PPAR AMPK
  4. Clitorin

Clitorin is an orally active flavonoid compound that can be isolated from Carica papaya. Clitorin is an inhibitor of EGFR (IC50: 89.58 nM) and aromatase (IC50: 77.41 nM). Clitorin has antioxidant and anti-tumor activities. In the DPPH and ABTS radical scavenging assays, Clitorin has IC50 values of 91.96 ppm and 250.45 ppm, respectively. In addition, Clitorin can regulate lipogenesis and fatty acid oxidation and can be used in the research of non-alcoholic fatty liver disease.

For research use only. We do not sell to patients.

Clitorin Chemical Structure

Clitorin Chemical Structure

CAS No. : 55804-74-5

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Customer Review

Based on 1 publication(s) in Google Scholar

Clitorin Related Antibodies

Top Publications Citing Use of Products

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EGFR/ErbB1/HER1 ErbB2/HER2 ErbB3/HER3 ErbB4/HER4

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CYP1 CYP2 CYP3 CYP4 CYP11 CYP17 Aromatase/CYP19A1 CYP26 CYP51 CYP1A2 CYP2D6 CYP2C9 CYP2C19 CYP3A4 CYP17A1

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PPARα PPARβ/δ PPARγ PPAR PPARδ

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NUAK1 NUAK2 AMPK AMPK α1β1γ1 AMPK α2β1γ1 AMPK α1β2γ1 AMPK α2β2γ1

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Clitorin is an orally active flavonoid compound that can be isolated from Carica papaya. Clitorin is an inhibitor of EGFR (IC50: 89.58 nM) and aromatase (IC50: 77.41 nM). Clitorin has antioxidant and anti-tumor activities. In the DPPH and ABTS radical scavenging assays, Clitorin has IC50 values of 91.96 ppm and 250.45 ppm, respectively. In addition, Clitorin can regulate lipogenesis and fatty acid oxidation and can be used in the research of non-alcoholic fatty liver disease[1][2][3].

IC50 & Target[1][2]

PPARα

 

Aromatase

77.41 nM (IC50)

In Vitro

Clitorin (24 h) is toxic to MCF-7 breast cancer cells, with an IC50 of 2.47 μM[1].
Clitorin (50-200 μM; 48 h) can reduce lipid accumulation, decrease the contents of triglycerides and total cholesterol, inhibit the mRNA levels of lipogenic genes, and enhance the mRNA levels of fatty acid oxidation genes in Oleic acid (HY-N1446)-stimulated HepG2 cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clitorin Related Antibodies

Real Time qPCR[2]

Cell Line: Oleic acid (HY-N1446)-stimulated HepG2 cells
Concentration: 50, 100 and 200 μM
Incubation Time: 48 h
Result: Inhibited the mRNA levels of SREBP1, ACC, FAS and HMGCR.
Enhanced the mRNA levels of CPT-1, PPARα, and AMPK.
In Vivo

Clitorin (10-20 mg/kg; oral administration; 4 weeks) has an improving effect in a mouse model of hepatic steatosis[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male C57BL/6J mice aged 6 weeks old treated western diet (WD) to induce hepatic steatosis[2]
Dosage: 10 and 20 mg/kg
Administration: Oral administration (p.o.); 4 weeks
Result: Sgnificantly alleviated body weight gain and hepatic steatosis features (NAFLD activity score, micro-, and macro-vesicular steatosis) in WD-induced hepatic steatosis mice.
Significantly decreased protein expressions of SREBP1, PPARγ and C/EBPα in WD-induced hepatic steatosis mice.
Significantly diminished the mRNA levels of SREBP1, ACC, FAS, and HMGCR and enhanced the mRNA levels of PPARαand CTP-1, as well as AMPK in the liver of WD-induced hepatic steatosis mice.
Molecular Weight

740.66

Formula

C33H40O19
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C(O[C@H]2[C@@H]([C@H]([C@H](O)[C@@H](CO[C@H]3[C@@H]([C@@H]([C@@H](O)[C@H](C)O3)O)O)O2)O)O[C@@]4([H])[C@@H]([C@@H]([C@@H](O)[C@H](C)O4)O)O)=C(C5=CC=C(O)C=C5)OC6=CC(O)=CC(O)=C61
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (135.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3501 mL 6.7507 mL 13.5015 mL
5 mM 0.2700 mL 1.3501 mL 2.7003 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (3.38 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (3.38 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.45%

SDS (252 KB)

COA (263 KB) HNMR (120 KB) CNMR (123 KB) RP-HPLC (244 KB) MS (99 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.3501 mL 6.7507 mL 13.5015 mL 33.7537 mL
5 mM 0.2700 mL 1.3501 mL 2.7003 mL 6.7507 mL
10 mM 0.1350 mL 0.6751 mL 1.3501 mL 3.3754 mL
15 mM 0.0900 mL 0.4500 mL 0.9001 mL 2.2502 mL
20 mM 0.0675 mL 0.3375 mL 0.6751 mL 1.6877 mL
25 mM 0.0540 mL 0.2700 mL 0.5401 mL 1.3501 mL
30 mM 0.0450 mL 0.2250 mL 0.4500 mL 1.1251 mL
40 mM 0.0338 mL 0.1688 mL 0.3375 mL 0.8438 mL
50 mM 0.0270 mL 0.1350 mL 0.2700 mL 0.6751 mL
60 mM 0.0225 mL 0.1125 mL 0.2250 mL 0.5626 mL
80 mM 0.0169 mL 0.0844 mL 0.1688 mL 0.4219 mL
100 mM 0.0135 mL 0.0675 mL 0.1350 mL 0.3375 mL
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Clitorin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Clitorin55804-74-5EGFRCytochrome P450Fatty Acid Synthase (FASN)Acetyl-CoA CarboxylaseHMG-CoA Reductase (HMGCR)PPARAMPKEpidermal growth factor receptorErbB-1HER1CYPsACC, Acetyl Coenzyme A Carboxylase Peroxisome proliferator-activated receptorsAMP-activated protein kinasehepatic steatosis modelMCF-7HepG2Inhibitorinhibitorinhibit

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