2. Epigenetics PI3K/Akt/mTOR Metabolic Enzyme/Protease
  3. AMPK Acetyl-CoA Carboxylase
  4. CAD031

CAD031 is an orally active AMPK/ACC1 signaling pathway activator and a derivative of the Alzheimer's disease (AD) targeted agent J147 (HY-13779) (more active than J147 in human neural stem cell assays). CAD031 can cross the blood-brain barrier, activate AMPK and inhibit ACC1, thereby increasing ac-CoA levels, improving mitochondrial function and reducing free fatty acid synthesis. CAD031 has neuroprotective, neurogenesis-promoting and memory-improving activities and can be used in the study of Alzheimer's disease and aging-related neurodegenerative diseases. CAD031 effectively enhances the memory of mice, improves dendritic structure, and stimulates cell division in the germinal zone of the brain of elderly mice.

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CAD031 Chemical Structure

CAD031 Chemical Structure

CAS No. : 2071209-49-7

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Based on 1 publication(s) in Google Scholar

CAD031 Related Antibodies

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NUAK1 NUAK2 AMPK AMPK α1β1γ1 AMPK α2β1γ1 AMPK α1β2γ1 AMPK α2β2γ1

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  • Purity & Documentation

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Description

CAD031 is an orally active AMPK/ACC1 signaling pathway activator and a derivative of the Alzheimer's disease (AD) targeted agent J147 (HY-13779) (more active than J147 in human neural stem cell assays). CAD031 can cross the blood-brain barrier, activate AMPK and inhibit ACC1, thereby increasing ac-CoA levels, improving mitochondrial function and reducing free fatty acid synthesis. CAD031 has neuroprotective, neurogenesis-promoting and memory-improving activities and can be used in the study of Alzheimer's disease and aging-related neurodegenerative diseases. CAD031 effectively enhances the memory of mice, improves dendritic structure, and stimulates cell division in the germinal zone of the brain of elderly mice[1][2][3].

In Vitro

CAD031 (100 nM; 7 d) significantly induces the mRNA expression of neurogenesis markers Nestin, Pax6, DCX, and Ki67 in human neural precursor cells (hNPCs) experiments; and 1 μM CAD031 inhibits Aβ-induced cell death in MC65 cells and glutamate-induced cytotoxicity in HT22 cells, exerting neuroprotective activity[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CAD031 Related Antibodies

RT-PCR[1]

Cell Line: Human embryonic stem cell-derived neural progenitor cells (Hues6 and H9)
Concentration: 100 nM
Incubation Time: 7 days
Result: Increased transcript levels of neurogenesis-related genes (Nestin, Pax6, DCX, Ki67) compared to control, while showing no significant effect on early stem cell markers (Sox1, Sox2). The effect was normalized to fibroblast growth factor (FGF).
In Vivo

CAD031 (200 ppm; dietary administration; daily; 6 months) improves fear conditioning and spatial pattern separation memory, increases neural precursor cell proliferation in the dentate gyrus and subventricular zone, without affecting Aβ plaque burden in the APPswe/PS1ΔE9 transgenic AD mouse model[1].
CAD031 (200 ppm; dietary administration; daily; 4 months) extends median lifespan, maintained mitochondrial gene expression and protein levels, reduces plasma free fatty acids, and activates the hepatic AMPK/ACC1 pathway in rapidly aging SAMP8 mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: APPswe/PS1ΔE9 Transgenic AD Mouse Model** Female APPswe/PS1ΔE9 transgenic mice (3 months old, weight not specified) + wild-type (WT) C57BL/6 controls[2]
Dosage: 200 ppm (premixed in diet)
Administration: Dietary administration, daily for 6 months
Result: Rescued context-dependent fear memory deficits in AD mice (increased freezing time) and restored the recognition index in a spatial pattern separation test.
Increased the number of BrdU+ cells in the dentate gyrus and BrdU+/DCX+ neural progenitors in the subventricular zone, while having no effect on Aβ plaque number or size.
Molecular Weight

404.31

Formula

C18H14F6N2O2
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(N(C1=CC=C(C)C=C1C)/N=C/C2=CC=CC(OC(F)(F)F)=C2)C(F)(F)F
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (247.33 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4733 mL 12.3667 mL 24.7335 mL
5 mM 0.4947 mL 2.4733 mL 4.9467 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.5 mg/mL (6.18 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 2.5 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: 2.5 mg/mL (6.18 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4733 mL 12.3667 mL 24.7335 mL 61.8337 mL
5 mM 0.4947 mL 2.4733 mL 4.9467 mL 12.3667 mL
10 mM 0.2473 mL 1.2367 mL 2.4733 mL 6.1834 mL
15 mM 0.1649 mL 0.8244 mL 1.6489 mL 4.1222 mL
20 mM 0.1237 mL 0.6183 mL 1.2367 mL 3.0917 mL
25 mM 0.0989 mL 0.4947 mL 0.9893 mL 2.4733 mL
30 mM 0.0824 mL 0.4122 mL 0.8244 mL 2.0611 mL
40 mM 0.0618 mL 0.3092 mL 0.6183 mL 1.5458 mL
50 mM 0.0495 mL 0.2473 mL 0.4947 mL 1.2367 mL
60 mM 0.0412 mL 0.2061 mL 0.4122 mL 1.0306 mL
80 mM 0.0309 mL 0.1546 mL 0.3092 mL 0.7729 mL
100 mM 0.0247 mL 0.1237 mL 0.2473 mL 0.6183 mL
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CAD031 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CAD0312071209-49-7CAD 031CAD-031AMPKAcetyl-CoA CarboxylaseAMP-activated protein kinaseACC, Acetyl Coenzyme A CarboxylaseAgingAlzheimer's diseaseJ147Memory enhancementNeurodegenerative diseaseNeurogenesisNeuroprotectionStem cellsInhibitorinhibitorinhibit

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