2. PI3K/Akt/mTOR
  3. mTOR
  4. C24-Ceramide

C24-Ceramide is an orally active competitive binding agonist of PIP4K2C (mTOR complex regulator), thereby activating the mTOR signaling pathway. At the same time, C24-Ceramide changes the membrane morphology by inducing the formation of a partially interlocked gel phase in the phospholipid bilayer. C24-Ceramide can promote the proliferation and migration of keratinocytes to accelerate skin wound healing and drive the proliferation and metastasis of gallbladder cancer cells. The level of C24-Ceramide in serum can be used as a diagnostic marker for gallbladder cancer.

For research use only. We do not sell to patients.

C24-Ceramide Chemical Structure

C24-Ceramide Chemical Structure

CAS No. : 34435-05-7

Size Price Stock Quantity
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg In-stock
100 mg   Get quote  
200 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of C24-Ceramide:

C24-Ceramide Related Antibodies

Top Publications Citing Use of Products

View All mTOR Isoform Specific Products:

View All Isoforms
mTOR mTORC1 mTORC2

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

C24-Ceramide is an orally active competitive binding agonist of PIP4K2C (mTOR complex regulator), thereby activating the mTOR signaling pathway. At the same time, C24-Ceramide changes the membrane morphology by inducing the formation of a partially interlocked gel phase in the phospholipid bilayer. C24-Ceramide can promote the proliferation and migration of keratinocytes to accelerate skin wound healing and drive the proliferation and metastasis of gallbladder cancer cells. The level of C24-Ceramide in serum can be used as a diagnostic marker for gallbladder cancer[1][2][3].

In Vitro

C24-Ceramide (100 μg/mL; 48 h) significantly promotes cell proliferation and migration in human keratinocytes (HaCaT)[2].
C24-Ceramide (500 μg/mL; 24 h) activates AKT and ERK1/2 signaling pathways[2].
C24-Ceramide (100 nM; 12 h) promotes cell proliferation and migration in gallbladder cancer cells (GBC-SD, NOZ) by binding to PIP4K2C and activating mTOR signaling, while C6-Ceramide competitively inhibited this effect[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

C24-Ceramide Related Antibodies

Cell Viability Assay[2][3]

Cell Line: HaCaT, GBC-SD, NOZ
Concentration: 100 μg/mL for HaCaT cells, 100 nM for GBC-SD, NOZ cells
Incubation Time: 24-48 h
Result: Promoted cell migration in HaCaT and GBC cells. Enhanced membrane flexibility that facilitates cell movement.
In Vivo

C24-Ceramide (4.4 μg/time; topical application; once daily; 10 days) accelerates wound closure, promotes epidermal and dermal regeneration and collagen deposition in the HR-1 hairless mouse skin wound model[2].
C24-Ceramide (10 mg/kg; dietary supplementation; once daily; 3 weeks) promotes the growth of gallbladder cancer (GBC-SD cells) in a nude mouse subcutaneous xenograft tumor model, as evidenced by increased tumor volume, weight, and the proportion of Ki-67 positive cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male HR-1 hairless mice (6-week-old, 18-20 g), full-thickness skin wound model[2]
Dosage: 4.4 μg C24-Ceramide per dose (formulated as C24-LNP in deionized water)
Administration: Topical application (10 μL of 1 mg/mL C24-LNP) daily for 10 days
Result: Significantly accelerated wound closure.
Enhanced re-epithelialization, increased fibroblast proliferation, and improved collagen deposition in the dermis, as confirmed by Masson’s trichrome staining.
Activated AKT and ERK1/2 signaling pathways in wound tissues, promoting cell proliferation and migration.
Animal Model: Nude mice subcutaneous xenograft model with GBC-SD cells[3]
Dosage: 10 mg/kg
Administration: Daily oral administration via dietary addition for 3 consecutive weeks
Result: Significantly increased tumor size and Ki-67 reactivity in xenograft sections, indicating enhanced cell proliferation.
Promoted hepatic metastasis, with higher incidence and size of metastatic lesions compared to control.
Activated mTOR signaling through binding to PIP4K2C, which was antagonized by C6-Ceramide co-treatment.
Molecular Weight

650.11

Formula

C42H83NO3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CCCCCCCCCCCCCCCCCCCCCCCC(N[C@@H](CO)[C@H](O)/C=C/CCCCCCCCCCCCC)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

Ethanol : 25 mg/mL (38.46 mM; Need ultrasonic)

THF : 25 mg/mL (38.46 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5382 mL 7.6910 mL 15.3820 mL
5 mM 0.3076 mL 1.5382 mL 3.0764 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.92%

SDS (393 KB)

COA (237 KB) HNMR (283 KB) RP-HPLC (222 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol / THF 1 mM 1.5382 mL 7.6910 mL 15.3820 mL 38.4550 mL
5 mM 0.3076 mL 1.5382 mL 3.0764 mL 7.6910 mL
10 mM 0.1538 mL 0.7691 mL 1.5382 mL 3.8455 mL
15 mM 0.1025 mL 0.5127 mL 1.0255 mL 2.5637 mL
20 mM 0.0769 mL 0.3846 mL 0.7691 mL 1.9228 mL
25 mM 0.0615 mL 0.3076 mL 0.6153 mL 1.5382 mL
30 mM 0.0513 mL 0.2564 mL 0.5127 mL 1.2818 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

C24-Ceramide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

C24-Ceramide34435-05-7mTORMammalian target of Rapamycinlipid membranesmembrane reorganizationInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
C24-Ceramide
Cat. No.:
HY-134508
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (393 KB) Français - FR (393 KB) Deutsch - DE (393 KB) Norwegian - NO (393 KB) Español - ES (393 KB) Swedish - SV (393 KB) Italian - IT (393 KB) Portuguese - PT (393 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.